| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 57.9% | ‑0.5 / 16.3 | 19 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (7/1); new base A (0/11); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTA > NZ_CP009273/4058879‑4059044 | ttAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATc > 1:13449/1‑90 (MQ=255)ttAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATc > 1:526762/1‑90 (MQ=255) cAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCAt > 1:161626/1‑90 (MQ=255) cAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCAt > 1:626940/1‑90 (MQ=255) tttCCGCGAGTCGAGAAAAAATCCGGTGAGTATATCGGTAAACGCGGAAAAAACCTTCCCCCCCAAAAAGATAACCCCGCCATAGTAAGc < 2:621846/90‑1 (MQ=255) ttcccGGGAGTAGAAAAAAAACCGGGGGAACATATGGGAAAACGGGAAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGc < 1:439293/87‑1 (MQ=255) ttcccGCGAGTCGAGAAAAAACCCGGTGAGCATATCGGAAAACGCTGAAAAAAACTTCCCCCCCAAAAAGATAATCCCGCCATAGTAAGc < 2:133003/87‑1 (MQ=255) gggagtcgagaAAAAACCCGGTGAGAATATCGGAAACCGCGGAAAAAACCTCCCCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCa < 2:311555/88‑1 (MQ=255) gcgAGTCGAAAAAAAACCGGGTGAAAATATGGGAAAACGCGGAAAAAACCTTCCCCACCAAAAAAATAACCCCGCCATAGTAAGCAGGCa < 1:425236/90‑1 (MQ=255) aaaaCCCGGTGACCATACCGGAAAACGGGGAAAAAACCTTCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCAt < 1:100832/90‑1 (MQ=255) aaaTCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACAAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 2:38589/90‑1 (MQ=255) ggTGAGCATATGGGTAAACGGGGAAAAAAACTCCGCCCCAAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata < 2:281244/90‑1 (MQ=255) cATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAATGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAttt > 2:394088/1‑90 (MQ=255) gcGGAAAACGGGTAAAAAAACTTCCCAACAAAAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 1:280857/89‑1 (MQ=255) tcccccacaaAAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 1:33844/86‑1 (MQ=255) tcccccacaaAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 1:183615/86‑1 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 1:294126/1‑90 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 1:571400/1‑90 (MQ=255) caaGAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTa < 1:445851/88‑1 (MQ=255) | TTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTA > NZ_CP009273/4058879‑4059044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |