| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,245,989 | 0 | C | T | 55.0% | ‑0.6 / 15.8 | 20 | E136K (GAA→AAA) | plsB | glycerol‑3‑phosphate 1‑O‑acyltransferase PlsB |
| Reads supporting (aligned to +/- strand): ref base C (1/8); new base T (11/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTAC > NZ_CP009273/4245913‑4246057 | cgACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGgcgcg < 1:196798/90‑1 (MQ=255) gACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGgcgcgc > 2:155271/1‑90 (MQ=255) gACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCCTTTTTACCCCCCGgcgcgc > 2:626421/1‑90 (MQ=255) aGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCa < 1:272555/90‑1 (MQ=255) caGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACAt < 1:458139/90‑1 (MQ=255) gTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTTCCCTTTTTTACCCCCCCGCGCGCGCCCCAACaacc > 1:220717/1‑87 (MQ=255) cAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCg < 2:570523/90‑1 (MQ=255) cAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCTCCCTTTTTTCCCCCCCGCGCGCGACCAAACaccaccc > 1:618376/1‑89 (MQ=255) cAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCGGCGCGCGGCCAAACATCACCg > 2:255053/1‑90 (MQ=255) gCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTCCTTTGCCTTTTTTTCCCCCCCGCGCGCGGCCAAAAaccacccgc > 1:370037/1‑87 (MQ=255) gCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACCCCCCCGCGCGCGACCAAAAAACACCGac > 1:224782/1‑90 (MQ=255) gCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCCCCCTTTTTTCCCCCCCGCGCGCGGCCAAAAATCAccccc > 1:356462/1‑87 (MQ=255) gAAAAATTTCTGTCCGCCGTTAAGCATACGCAGCGGCGGGTTCCCTTCGCCTTTTTCCCGCCCCGGCGCGCGACCAAACATCACCGacac < 1:588873/90‑1 (MQ=255) aaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCCGCGCGCGGCCCAACCACACCCACACCgg > 2:375025/1‑90 (MQ=255) gTTAAGCATACGCAGCGGCGGGTTTCCTTCGCCTTTTTTACGCCCCCGCGCGCGACCCAACAACACCCGCACTGGCCCCCCCTGCACCTc > 1:64395/1‑90 (MQ=255) cATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAtt < 1:255053/90‑1 (MQ=255) aTACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAttt < 1:227237/90‑1 (MQ=255) tACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTg > 2:100464/1‑90 (MQ=255) gcggcgGGTTCACTTCGCCTTTTTTACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTTGGGTGc > 2:148217/1‑90 (MQ=255) gcggGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTAc < 1:155271/90‑1 (MQ=255) | CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTAC > NZ_CP009273/4245913‑4246057 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |