| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 172,755 | 0 | A | G | 57.9% | 1.4 / 16.3 | 19 | T388A (ACG→GCG) | clcA | H(+)/Cl(‑) exchange transporter ClcA |
| Reads supporting (aligned to +/- strand): ref base A (1/7); new base G (11/0); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTG‑CCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGG > NZ_CP009273/172675‑172833 | tGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTa < 2:211702/90‑1 (MQ=255) gCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTG‑CCGGAATGGGGGc < 2:339981/90‑1 (MQ=255) cTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTTCTATTT‑CCGGGATgggggga > 2:58375/1‑88 (MQ=255) cTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTTCTATTG‑CCGGGATGGGGGCa > 1:215590/1‑90 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGGGGTTTCTTTTG‑CCGGGATGGGGGCAtt > 1:563072/1‑90 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTTCTATTG‑CCGGGATGGGGGGAtt > 2:206922/1‑90 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGCTATTT‑CCGGGATGGGGGGAtt > 2:124256/1‑90 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGCTATTG‑CCgg > 1:505793/1‑78 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTATTG‑CCGGGATGGGGGCAtt > 1:546671/1‑90 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTG‑CCgg < 2:505793/78‑1 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGGTTCCGCAATATCACCTTGAGGCGGGGGGGTTTTCTTTTGTCCGGATTGGGGGctt > 1:329851/1‑88 (MQ=255) cGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGGTTGCTATTG‑CCGGAATGGGGGCATTACTGGCGGGATCTATTTGCGCGcc > 1:72236/1‑90 (MQ=255) ttGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTG‑CCGGAATGGGGGCATTACTGGCGGCACCTATTCGCGCGCCGt < 2:425499/90‑1 (MQ=255) ttCCGCAATATCACCTTGAGGCGGGGGGGGTTGCTATTG‑CCGGGATGGGGGGATTTCTTGGGGGCTCTATTTGCGCGCCCTTTACggggg > 1:282492/1‑89 (MQ=255) ttCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTATTG‑CCGGGATGGGGGGATTACTTGCGGGATCTATTTTCGCGCCGGTTACggggg > 1:74228/1‑89 (MQ=255) tCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTG‑CCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGAt < 1:434912/90‑1 (MQ=255) atatCACCTTGAGGCGGGGACGTTTGCTATTG‑CCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCAttct < 2:563072/90‑1 (MQ=255) aCCTTGAGGCGGGGACGTTTGCTATTG‑CCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCAttctggttc < 2:175799/90‑1 (MQ=255) ttGAGGCGGGGGCGTTTGCTATTG‑CCGGGATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGGTCAttttggttttgg > 1:335467/1‑90 (MQ=255) | TGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTG‑CCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGG > NZ_CP009273/172675‑172833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |