| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,884 | C→A | P21Q (CCG→CAG) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,884 | 0 | C | A | 90.9% | 51.6 / ‑1.9 | 22 | P21Q (CCG→CAG) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (4/16); total (5/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
TTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGA > NZ_CP009273/49802‑49965 | tttttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAAc < 2:299100/90‑1 (MQ=255) tCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACctgcctgc < 2:149621/90‑1 (MQ=255) tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGcc < 1:167463/90‑1 (MQ=255) aaTGATCAGTCTGATTGCGGCGTTAGCGGTGGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTg < 1:426160/90‑1 (MQ=255) tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa < 1:160021/90‑1 (MQ=255) gtttGCGGCGTTAGCGGTAGAGCGCGTTACCGGCATGAAAACCGCCATGCAGGGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:486649/88‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:185402/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 2:154320/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:561037/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:648399/90‑1 (MQ=255) gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACAcc > 2:510673/1‑90 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt < 1:540858/90‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa < 2:640549/77‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGGTTa > 1:640549/1‑77 (MQ=255) aGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt < 1:91228/90‑1 (MQ=255) tCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa > 1:42479/1‑69 (MQ=255) tCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 2:42479/69‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 1:195905/1‑90 (MQ=255) ttATCGGAATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg < 1:645623/90‑1 (MQ=255) aTGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 1:439004/90‑1 (MQ=255) tGGAAACCGCCATGAAGTGGACCCTCCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa < 1:290452/90‑1 (MQ=255) cGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa > 2:450478/1‑90 (MQ=255) | TTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGA > NZ_CP009273/49802‑49965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |