| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,184,613 | A→T | I207N (ATC→AAC) | phoQ ← | two‑component system sensor histidine kinase PhoQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,184,613 | 0 | A | T | 96.4% | 92.7 / ‑3.1 | 28 | I207N (ATC→AAC) | phoQ | two‑component system sensor histidine kinase PhoQ |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (12/15); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01 | |||||||||||
CTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGATCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAATGGTAT > NZ_CP009273/1184536‑1184697 | cttcCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAAcagca > 1:276859/1‑90 (MQ=255)cttcCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAAcagca > 1:626994/1‑90 (MQ=255) cgGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTgag < 2:276859/90‑1 (MQ=255) ggACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTgaga < 1:8792/90‑1 (MQ=255) ttcttTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGcac > 2:553610/1‑90 (MQ=255) tGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGAt < 2:584530/90‑1 (MQ=255) gggCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAAcc < 2:626994/90‑1 (MQ=255) gggCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAAcc > 1:176354/1‑90 (MQ=255) gggCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAAcc < 1:582341/90‑1 (MQ=255) ggCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCa < 1:553610/90‑1 (MQ=255) aTGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAg < 1:123390/90‑1 (MQ=255) gggCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCa < 1:505857/80‑1 (MQ=255) gggCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCa > 2:505857/1‑80 (MQ=255) gggCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGAcc > 1:165425/1‑90 (MQ=255) gggCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGAcc < 2:136705/90‑1 (MQ=255) gTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAg < 1:135116/83‑1 (MQ=255) gTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAg > 2:135116/1‑83 (MQ=255) tAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCAtata < 1:11788/90‑1 (MQ=255) aCTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGa < 1:388657/90‑1 (MQ=255) ccaccaGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTgaga > 2:91096/1‑53 (MQ=255) ccaccaGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTgaga < 1:91096/53‑1 (MQ=255) ccaccaGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATACAGGAACt > 1:148390/1‑90 (MQ=255) aGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTa > 1:445988/1‑90 (MQ=255) gcggcgACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGc < 1:135408/90‑1 (MQ=255) cacaGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGa > 2:509075/1‑71 (MQ=255) cacaGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGa < 1:509075/71‑1 (MQ=255) gcGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAATGGTAt > 2:381883/1‑90 (MQ=255) gcGGGATCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAATGGTAt > 2:410378/1‑90 (MQ=255) | CTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGATCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAATGGTAT > NZ_CP009273/1184536‑1184697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |