Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,743,316	T→G	V167V (GTA→GTC)	ydhT ←	protein YdhT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,743,316	0	T	G	100.0%	32.4 / NA	11	V167V (GTA→GTC)	ydhT	protein YdhT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/6);  total (5/6)

TTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCA  >  NZ_CP009273/1743240‑1743391                                                                             |                                                                            ttGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGGACTCGCATTCCgg                                                                >  1:386206/1‑90 (MQ=255)                     tGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGGACTCGCATTCCGGTATTCGCAATCCGACTGCTa                                            <  1:395211/90‑1 (MQ=255)                     tGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGGACTCGCATTCCGGTATTCGCAATCCGACTGCTa                                            <  2:470236/90‑1 (MQ=255)                                     tACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGGACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTa                            >  1:205595/1‑90 (MQ=255)                                      aCTGGCGACATGACACCGTTCTCCCAATGTACAAACGCGGACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTAt                           <  1:442221/90‑1 (MQ=255)                                      aCTGGCGACATGACACCGTTCACCCAATGTACAAACGCGGACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTAt                           >  1:82640/1‑90 (MQ=255)                                            gACATGACACCGTTCACCCAATGTCCAAACGCGGACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCga                     <  1:393470/90‑1 (MQ=255)                                                       gTTCACCCAATGTACAAACGCGGACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCgg          >  2:422858/1‑90 (MQ=255)                                                               aaTGTACAAACGCGGACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCa  >  2:452882/1‑90 (MQ=255)                                                                          gcgGACTCGCATTCCGGTATTCGCAATCCGACTGCTa                                            <  2:423205/37‑1 (MQ=255)                                                                          gcgGACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGtt        <  2:375079/73‑1 (MQ=255)                                                                             |                                                                            TTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCA  >  NZ_CP009273/1743240‑1743391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑