| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,356,900 | 0 | T | C | 53.8% | 5.3 / 13.8 | 13 | G71G (GGA→GGG) | yfaY | nicotinamide mononucleotide deamidase‑related protein YfaY |
| Reads supporting (aligned to +/- strand): ref base T (6/0); new base C (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCAC > NZ_CP009273/2356811‑2356986 | tCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTggtcggt > 1:288273/1‑90 (MQ=255) gggCTTCATGCACCCCCGGCCCTCCCCTTTTTGCTGCCGCGGGGGCGAGGGCGCTAAAATCACCGCGGGTCGGCCCCAGCCCGCCGTTaa < 2:130129/89‑1 (MQ=255) ggCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAAc > 2:212174/1‑90 (MQ=255) ctccATGCACCCCCAGGCCTTCCCTTTTTGCTGTCGCGGCGGGGAGTGCGCTTAAATCATCGCGGGTGGGCCCCAGCCCGCCGTTAACGa < 1:154353/87‑1 (MQ=255) cagcaCCAGCCCTTCCCTTTTTGCTGCCGCAGGGGCGAGTGCGTTTAAATCATCGCGGGTGGGCCCCAGCCCGCCGTTAACGATCAGcac < 1:212174/90‑1 (MQ=255) gcaCCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACAt > 1:275137/1‑90 (MQ=255) cggcccTTCCCTTTTTGCTGTCGCGGGGGGGGGTGCGTTTAAATCACCGCGGGTCGGCCCCACCCCGCCGTTAACGATCAGCACATCGGc < 1:375984/86‑1 (MQ=255) aGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCg > 1:256015/1‑90 (MQ=255) cctttttGCTGCCGCGGGGGCGGGTGCGCTTAAACCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACt < 1:208968/87‑1 (MQ=255) tttGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGt > 1:176567/1‑90 (MQ=255) gTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCga > 1:291234/1‑90 (MQ=255) gcggcgAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATg > 2:82014/1‑90 (MQ=255) tcatcGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAgg < 1:300257/90‑1 (MQ=255) ccgcgcgGGTCCGCCCCCCCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGt < 2:58168/87‑1 (MQ=255) cggccCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCAc < 1:97803/90‑1 (MQ=255) | TCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCAC > NZ_CP009273/2356811‑2356986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |