| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,832,718 | 0 | T | G | 53.3% | 12.3 / 13.7 | 15 | intergenic (‑95/‑165) | ascG/ascF | DNA‑binding transcriptional regulator AscG/PTS cellobiose/arbutin/salicin transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (8/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTC > NZ_CP009273/2832635‑2832804 | cTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTCAGGTGACCCGGTTccca > 2:322531/1‑90 (MQ=255) cAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTTAGGTGACCCGTCTGACAAAAATaaaa > 1:339226/1‑90 (MQ=255) cGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAc < 1:164154/90‑1 (MQ=255) tgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGACCGGGTTCACAAATATAAAAAAAGAACAATTc > 2:12420/1‑90 (MQ=255) tACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAGTATAAAAAATGAACAATTCAc < 1:231858/90‑1 (MQ=255) tACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAc < 2:310055/90‑1 (MQ=255) cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAATATTTAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTTActc > 2:349331/1‑90 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGACCGGGTTCACAAATATAAAAAAAGAAAAATTTActctc > 2:170377/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAAAATTCAGGGGACCGGGTTCACAAATATAAAAAAAGAAAAAATCACTCTCTTGCt > 2:56938/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt > 1:62175/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAAAAATTTACTCTCTTGCt > 1:290395/1‑90 (MQ=255) tGTCCTTGCACAATCGGCGGGGAAAAAATTCAGGGGACCGGGTTCACAAATATAAAAAATGAAAAATTTACTCTCTTGGTTTTTTTGTGa > 2:113173/1‑90 (MQ=255) aaTCGGCGGGGAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCAt > 2:320017/1‑90 (MQ=255) aaaaTATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaa < 2:79460/90‑1 (MQ=255) cGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTc < 2:145006/90‑1 (MQ=255) | CTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTC > NZ_CP009273/2832635‑2832804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |