| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,571,661 | 0 | A | C | 53.8% | 11.1 / 15.0 | 13 | G142G (GGT→GGG) | gntR | gluconate operon transcriptional repressor GntR |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAG > NZ_CP009273/3571582‑3571739 | gTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACtt > 1:193243/1‑90 (MQ=255)gTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACtt > 2:330696/1‑90 (MQ=255) aaaGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCtt > 1:280149/1‑90 (MQ=255) tATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAgg > 2:200767/1‑90 (MQ=255) tATCAAAACCGACGGCGATATCAAAGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAgg > 2:13620/1‑90 (MQ=255) aagccGAGGGCAATATAAAGGGTGGGCGATTTGCTGTCAATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTgcgc < 1:395123/87‑1 (MQ=255) aacccGGCGGCGATACAAGGGCGTGGCCCTTTGCTGCCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTgcgc < 1:182683/87‑1 (MQ=255) aacccGAGGGGGATATCAGGGCATGGCGATTTGCTGCCCATCAGTTCCACCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTgcgc < 1:125464/87‑1 (MQ=255) cccGACGGCGATATAAAGGCTTGGCGCCTTGCTGCCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 1:109999/89‑1 (MQ=255) aCCGACGGCAATATCAAGGCATGGCGTTTTGCTGTCCATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 1:126859/90‑1 (MQ=255) ggCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAgg > 1:215488/1‑90 (MQ=255) cATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 1:99549/90‑1 (MQ=255) cccagGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCATCGATATTCCAGgag < 2:74143/89‑1 (MQ=255) | GTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAG > NZ_CP009273/3571582‑3571739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |