| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,679,388 | 0 | T | C | 56.2% | 6.4 / 14.4 | 16 | E1049G (GAG→GGG) | bcsC | cellulose synthase complex outer membrane protein BcsC |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base C (0/9); total (4/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCC > NZ_CP009273/3679320‑3679471 | gATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTgc > 2:74232/1‑90 (MQ=255) ttCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTACCACGACCAGTTTTCCGTgcgc < 1:171298/90‑1 (MQ=255) aaCGCGGCATGTTTTTGGTGCGTGAAGGCGCCCACGAGCCAGACGCCCCCAGCCCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCa < 1:102467/90‑1 (MQ=255) gggCATGGTTTTGGTGCGTGAATGCGCCCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCCCATCACCgg < 2:356535/89‑1 (MQ=255) cATGTTTTTGGTGCGTGAATGCGCCCCCGAGCCAGACGCCCCCAGCCCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTAt < 1:1986/90‑1 (MQ=255) tGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATgg > 1:87420/1‑90 (MQ=255) gTGAGTGCCCCCCCGACCCAGCCCCCCCCAGCCCCCCCGCCCTTTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGt < 2:87420/90‑1 (MQ=255) gTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGt > 1:192624/1‑90 (MQ=255) cccGAGCCAGACGCCCCCCGCCCCCACACCTTTTTTTCCGGGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGa < 2:375481/88‑1 (MQ=255) ccgcgCCAGCCCCCCCCACCCCCCCCGCCCTTTTTTCCGGGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGAc < 2:66942/86‑1 (MQ=255) ccGACCCAGACCCCCCCCGCCCCCACGACCGGTTTTCCGGGCCCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGAc < 2:358820/89‑1 (MQ=255) aCGAGCCAGACGCCCCCAGCCCCCACGACCATTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGAc < 1:70626/90‑1 (MQ=255) cccccccACCCCCCACGACCGGTTTCCGGTGCTCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc < 1:350962/86‑1 (MQ=255) cACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGa < 1:64632/61‑1 (MQ=255) cACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGa > 2:64632/1‑61 (MQ=255) cccACCCCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGccc < 1:157787/90‑1 (MQ=255) | GATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCC > NZ_CP009273/3679320‑3679471 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |