| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 536,014 | 0 | T | G | 56.2% | 3.4 / 12.7 | 16 | intergenic (+50/‑7) | allB/ybbY | allantoinase AllB/uracil/xanthine transporter |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base G (9/0); total (13/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAAC > NZ_CP009273/535931‑536092 | gAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGGTTAGGGGTGttt > 2:89038/1‑90 (MQ=255) ttAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCCTTCTCCGGGTTAGGGGGGTTTTTGTTTAATTTTTCAGt > 2:148256/1‑90 (MQ=255) tCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTTAGGGGGGTTTATGTTCAATTTTTCAGTCAGCcgc > 1:364956/1‑90 (MQ=255) tAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTTAGGGGTGTTTTTGTTTAATTTTTCAGGCAGCCGCGAAAGcc > 1:265571/1‑90 (MQ=255) aaTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTTTGTTCAATTTTGCAGTCAGCCGCGAAAGcc > 1:8954/1‑89 (MQ=255) aaTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGcc < 2:8954/89‑1 (MQ=255) ggCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTTCAGTCAGCCGCGAAAGCCt > 1:84812/1‑85 (MQ=255) ggCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCt < 2:84812/85‑1 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGGATTCTCCCGTTTAGGGGTGGTTTTGTTTTATTTTTGAGTCAGCCCCGGAAGCCCGTTTTCTggg > 2:181961/1‑89 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTAAGGGGGGGTTTTGTTTCATTTTTCAGTCAGCCGCGAAAACCTGTTATCAGGa > 1:343976/1‑90 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTTAGGTGGGTTTTTTTTTAATTTTTTAGGCGGCCGCGAAAGCCTGTTATCAggg > 2:261903/1‑89 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGGGTTTTTGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGa > 1:35611/1‑90 (MQ=255) gTCCTTGCGGCGGGCATTCTCCCGTTTAGGGGTGTTTTTGTTCAATTTTGCAGTCCACCCCGGAAGACTGGTTTAAGGGTTTTAGAGGtt > 1:327862/1‑90 (MQ=255) tCCTTGCGGCGGGCGTTCTTCGGGTTAGGGGGGTTTTTATTTTATTTTTCAGTCAGGCCCGGAAACCCGTTATCAAGATTTCAGCGGgtt > 2:330971/1‑90 (MQ=255) gggCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTTTTTAttt > 2:90485/1‑90 (MQ=255) tcCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAac < 1:188483/90‑1 (MQ=255) | GAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAAC > NZ_CP009273/535931‑536092 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |