Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,378	T→C	M1A (GTG→GCG) †	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,378	0	T	C	100.0%	36.9 / NA	12	M1A (GTG→GCG) †	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/5);  total (7/5)

GGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTG  >  NZ_CP009273/1613331‑1613460                                                |                                                                                   gggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTggg                                          >  1:329399/1‑90 (MQ=255)     aaTATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGc                                      <  1:323907/90‑1 (MQ=255)              cccTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCat                             <  2:329399/90‑1 (MQ=255)                cTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCatat                           >  1:374272/1‑90 (MQ=255)                cTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCatat                           >  2:354480/1‑90 (MQ=255)                         aTTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCa                  <  1:161820/90‑1 (MQ=255)                               tGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAgaagaa            >  2:248026/1‑90 (MQ=255)                                gCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATc                   <  1:253638/82‑1 (MQ=255)                                gCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATc                   >  2:253638/1‑82 (MQ=255)                                    gggCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATc       >  2:191476/1‑90 (MQ=255)                                       cAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGcc    <  2:16836/90‑1 (MQ=255)                                         aCTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTg  >  2:50305/1‑90 (MQ=255)                                                |                                                                                   GGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTG  >  NZ_CP009273/1613331‑1613460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑