Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,902,956	A→T	V23E (GTA→GAA)	mgrB ←	PhoP/PhoQ regulator MgrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,902,956	0	A	T	100.0%	38.9 / NA	12	V23E (GTA→GAA)	mgrB	PhoP/PhoQ regulator MgrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (7/5);  total (7/5)

ACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCT  >  NZ_CP009273/1902885‑1903042                                                                        |                                                                                       acGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATTCCTGCGCCCAAAgcagca                                                                      >  1:130765/1‑90 (MQ=255) acGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATTCCTGCGCCCAAAgcagca                                                                      >  1:183039/1‑90 (MQ=255)    ggATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATTCCTGCGCCCAAAGCagcaagc                                                                   >  1:245884/1‑90 (MQ=255)    ggATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATTCCTGCGCCCAAAGCagcaagc                                                                   <  2:280442/90‑1 (MQ=255)                   tGGCACAAATTCCGCTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATTCCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGa                                                    <  1:144671/90‑1 (MQ=255)                   tGGCACAAATTCCGCTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATTCCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGa                                                    <  2:15348/90‑1 (MQ=255)                                 cTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATTCCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGAcc                                      <  1:148125/90‑1 (MQ=255)                                  tGAAAAATTGTACATCCTGATCGCACATCATGTTGAATTCCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGAccc                                     >  2:146307/1‑90 (MQ=255)                                             aCATCCTGATCGCACATCATGTTGAATTCCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACttt                          >  1:264844/1‑90 (MQ=255)                                                   tGATCGCACATCATGTTGAATTCCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACt                    >  2:131818/1‑90 (MQ=255)                                                                tGTTGAATTCCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt       >  2:62081/1‑90 (MQ=255)                                                                     aaTTCCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacct  <  1:233856/90‑1 (MQ=255)                                                                        |                                                                                       ACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCT  >  NZ_CP009273/1902885‑1903042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑