| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,407,992 | 0 | A | T | 53.8% | 5.2 / 16.9 | 13 | Y2* (TAT→TAA) *65K (TAA→AAA) | rcbA ralR | double‑strand break reduction protein RcbA type I toxin‑antitoxin system endodeoxyribonuclease toxin RalR |
| Reads supporting (aligned to +/- strand): ref base A (0/6); new base T (7/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC > NZ_CP009273/1407905‑1408067 | gCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTtata < 1:667003/90‑1 (MQ=255) ttCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAcc < 2:52792/90‑1 (MQ=255) ttAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCa < 1:682036/90‑1 (MQ=255) gTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAATTAAAACCCCCcttt > 2:123221/1‑89 (MQ=255) gATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATATTAACACCCCCctttttttt > 2:576672/1‑90 (MQ=255) ttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGGAATTTTTTTTATTAAAACCTCCCTTAtttttttta > 1:876366/1‑87 (MQ=255) cAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTAtt < 1:123221/90‑1 (MQ=255) gTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGGGATTTTTTTAATGAAAAACACCCTTTTTTTTTTCAGTTGGTTGtttttttc > 1:834217/1‑88 (MQ=255) gTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTCATGAACACCCCCACTTTTTTTTTCATTGGGTCGTTTTTTTCTTCtttttag > 1:878910/1‑86 (MQ=255) gTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTAATGAAAACCCCCCTTTTTTTTTTCAGTGGGTTGTTTTTTTCATCttttgtg > 2:523234/1‑86 (MQ=255) gTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAATGAACACCCCCCTTATTTTTTTCCGTGGGTCGTTTTTTTCCTCTTTTGAg > 2:218336/1‑90 (MQ=255) tGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGt < 2:876366/90‑1 (MQ=255) ttttCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTc < 1:677405/90‑1 (MQ=255) gTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTccacc < 1:576672/90‑1 (MQ=255) | GCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC > NZ_CP009273/1407905‑1408067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |