| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,679,006 | 0 | A | C | 58.3% | 6.9 / 10.9 | 12 | D164A (GAC→GCC) | tus | DNA replication terminus site‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/7); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAG > NZ_CP009273/1678924‑1679093 | gcgGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGcc > 1:275218/1‑90 (MQ=255) gTGCCTCGTCTTTCGCGGGGGCTGACCCCCTTTAATGCTCCCCGCCCCCTCCCCTTCCGGCCCGCCCCCGCCACTTTACCCTttggttgg < 1:828863/90‑1 (MQ=255) gTGCATCGTCTTTTGCGGGGGCTGATCCCCCTTAATGCTTCCCGCCCGCTCACCGTTCTGCACGCCCCCGCCACTTTACGCTttggttgg < 1:27696/90‑1 (MQ=255) gTGCATCGTCTTTTGCCGGGGCTGCCCCCCTTTAATGCTTCCCGCCCGCCCCCCGTTCTGCACGCCCCCGCCACTTTACGCTttggttgg < 1:354207/90‑1 (MQ=255) gTGCATCGTCTTTTGCCGGGGCTGACCCCCCTTAATCCTTCCCGCCCGCTCCCCGTTCTGCCCCCCCCCGCCACTTTACGCTttggttgg < 2:657179/90‑1 (MQ=255) cccTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTAc > 1:395219/1‑90 (MQ=255) cTTAATGCTTCCCGCCCGCTCCCCTTTCTGCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACAt < 2:544586/90‑1 (MQ=255) ttAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATc > 1:510596/1‑90 (MQ=255) tcccGCACGCTCCCCTTTCTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGaa < 2:275218/88‑1 (MQ=255) ccGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGt > 1:236032/1‑90 (MQ=255) cttttCTGCCCACCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGc < 1:526909/87‑1 (MQ=255) cGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAg > 2:450924/1‑90 (MQ=255) | GCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAG > NZ_CP009273/1678924‑1679093 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |