| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,414,922 | 0 | A | C | 57.1% | 4.3 / 14.3 | 14 | Y40S (TAC→TCC) | folX | dihydroneopterin triphosphate 2'‑epimerase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCA > NZ_CP009273/2414839‑2415003 | ccTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCccgc > 2:464948/1‑90 (MQ=255) cgaaaTAAAGGAAAAAAAAATAAACACCCGTCAGGATTTTTTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGa < 2:273461/87‑1 (MQ=255) caaaaTAAAGGAAAAAAAAATAAACACCCGCCGGGATTTTTTAAACAATGTGCCGACCCCCACCCCCGCCGATAAAGCGCGCACTAGCGa < 1:169131/87‑1 (MQ=255) gaaaTTAAGAAAAAAAAAATTAACACCCGTAAGGTTTTTTTTATCAATGTGCCGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGaa < 2:506444/88‑1 (MQ=255) aaaTAAAGAAAAAAAAAATAAACACCCGTCAGGATATTTTTATAAAGGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAg < 2:573920/89‑1 (MQ=255) aaGAAAAAAAAAATAAACACCCGTCAGATTTTTTTTATCATGGTGACGCCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATc < 2:200020/90‑1 (MQ=255) agaagaaATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGa > 1:200020/1‑90 (MQ=255) taaacaccCGTAAGGATTTTGTTATAAATGTGACGTCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGa < 2:368802/88‑1 (MQ=255) gTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCa > 1:885578/1‑90 (MQ=255) atTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACg > 1:793297/1‑90 (MQ=255) tttATAATTGGGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGaaa < 2:138957/89‑1 (MQ=255) tttAACAATGTCACCACCCCCCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGaaa < 1:674234/89‑1 (MQ=255) aTCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAAc > 1:735025/1‑90 (MQ=255) tACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCa > 2:765386/1‑83 (MQ=255) | CCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCA > NZ_CP009273/2414839‑2415003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |