| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,957 | 0 | A | T | 54.2% | 13.6 / 31.6 | 24 | I17F (ATT→TTT) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (2/9); new base T (13/0); total (15/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGT > NZ_CP009273/3175870‑3176041 | aCGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGtttttt > 2:326938/1‑90 (MQ=255) cGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGGCACGTTTTTTg > 1:870907/1‑90 (MQ=255) aTCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCGCGTTTTTTGgc > 1:772591/1‑90 (MQ=255) aGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTTGCGCGTTTTTCg > 2:270879/1‑90 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGTTTTTTTGGGCGGTTTTCGGCGt > 1:43061/1‑90 (MQ=255) aTTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTtc‑t‑c < 2:624190/90‑1 (MQ=255) ttATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTTGGGCGGTTTTCGGCGGTC‑T‑C‑gg > 2:162934/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGCGCCCCGGTTTTTGGGGCGGTTTTCGGCGTTC‑TCC‑GGGCAAAAAc > 2:430536/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCCTTTTTTTGGGCGTTTTTCCGCGTTC‑T‑C‑CGGCaaaaaac > 2:901687/1‑88 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTTGGGCGTTTTTCGGCGTTC‑T‑C‑GGGCaaaaaac > 2:481105/1‑88 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGGCCCGTTTTTTTGCGCGTTTTTCCGCGTTT‑T‑CGGGGCAAAAACCC‑CGAAcc > 2:422151/1‑90 (MQ=255) cAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTTGGGCGGTTTTCCGCGGTC‑T‑C‑GGGCAAAAAACCCCGAAccccc > 2:799744/1‑87 (MQ=255) ttCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGTGCGGTTCTTTGCGGTCTT‑C‑GGCCAAAAACCC‑CGAACCGCTTTCCGGCGgttt > 1:908094/1‑90 (MQ=255) tGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGc < 2:43061/90‑1 (MQ=255) gACCATACTGGCGGGGGGCGCCACGGTTTTTTGGGCGGTTTTCCGCGTTT‑T‑C‑CGGCAAAAAACCCCCAACCCCCTACTGGCGGTTTTGCt > 1:634429/1‑90 (MQ=255) gACCATACTGGCGGGGGCAGCCCCGGTTTTTGGGGCGTTTCTCGGCGTTC‑T‑C‑GGGGAAAAAACCCCCAACCCCTTACTTGCGGTTTTGCt > 1:269345/1‑90 (MQ=255) aCCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 1:830712/90‑1 (MQ=255) aCCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 2:908094/90‑1 (MQ=255) aTACTGGCGGGGGCAGCCACGTTTTTTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGt > 2:46697/1‑90 (MQ=255) ggCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgc < 1:446482/90‑1 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:176511/90‑1 (MQ=255) ggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCggg < 1:901687/90‑1 (MQ=255) aGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 2:42831/90‑1 (MQ=255) gCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGTc > 1:61676/1‑90 (MQ=255) cGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGt < 2:61676/90‑1 (MQ=255) | ACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGT > NZ_CP009273/3175870‑3176041 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |