| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,259,543 | 0 | T | G | 52.9% | 15.7 / 18.0 | 17 | F68V (TTT→GTT) | aphA | acid phosphatase AphA |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base G (9/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAG > NZ_CP009273/4259457‑4259612 | ggCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTg > 1:874356/1‑90 (MQ=255) aCAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGa > 2:402867/1‑90 (MQ=255) ggCACCCATTCATTGGGTTTCGGTCGCAAAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGacac < 2:290378/90‑1 (MQ=255) aCCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGt < 1:882369/90‑1 (MQ=255) ccATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGGGGGGGTTGATATCGATGACACGGGAc > 1:743479/1‑90 (MQ=255) ttcattGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACttt > 2:639803/1‑90 (MQ=255) cattGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACttttt > 2:400232/1‑90 (MQ=255) gggTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCa > 1:224061/1‑90 (MQ=255) gggTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGGGGGGTTTGATATCGATGACACGGGACTTTTTTTCa > 1:793248/1‑90 (MQ=255) ttGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGcgcg < 1:639803/90‑1 (MQ=255) aGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCaaaaaa > 2:123065/1‑90 (MQ=255) gCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACCGGACTTTTTTTCAGTCCGGGCTTTTGGGCCGGCcaaaaaa > 1:193320/1‑90 (MQ=255) gggCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCg < 2:874356/90‑1 (MQ=255) gCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGcc > 2:194876/1‑90 (MQ=255) cGTCCGCCAATGGCGGTGGGGGTTTATATCGATGACACGGGACTTTTTTTCAGTCCGGGCTTCTGGCGCGGCAAAAAAAACTTCTCGCCa > 1:917124/1‑90 (MQ=255) gTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAg < 1:758729/90‑1 (MQ=255) gTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTTCAGTCCGGGCTTCTTGCGCGGCAAAAAAACCTTCTCGCCAg > 1:537034/1‑90 (MQ=255) | GGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAG > NZ_CP009273/4259457‑4259612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |