| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,290,979 | 0 | T | G | 52.9% | 13.7 / 20.1 | 17 | T639T (ACA→ACC) | mdtO | multidrug efflux transporter permease subunit MdtO |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (9/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGA > NZ_CP009273/4290896‑4291063 | gTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTctct > 1:822976/1‑90 (MQ=255)gTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGctct > 2:326732/1‑90 (MQ=255) ccAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTc < 2:397507/90‑1 (MQ=255) tCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTCAAGc > 2:308268/1‑90 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTTAAGCGttt > 1:583359/1‑89 (MQ=255) aaGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTAtt < 2:822976/90‑1 (MQ=255) gggCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCCAGCGTTTTTTtag > 2:211248/1‑90 (MQ=255) ttAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAg < 1:919249/90‑1 (MQ=255) aaGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTAt < 2:768960/90‑1 (MQ=255) aaGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTAt < 2:527481/90‑1 (MQ=255) aGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTATTTTAGGAGAACGGCTGAGGGGGGTTGCCAGACCGGCAGCGTAtt > 2:106786/1‑90 (MQ=255) aTGGCCTGAGAGGCGGGGGTCTCTTCAAGGGTTATTTGTGGGGAACGGGTGGGGGGGGGTGGCCGACCCGGAGCGTGTTTTTTCAGGGCg > 1:325333/1‑90 (MQ=255) tGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTTTTTTAGGAGAACGGGTGAGTGCGGGTGCCAGACCGGCAGCGGATTTTTCCAgggggg > 2:2080/1‑87 (MQ=255) gagaGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCaa < 1:658444/90‑1 (MQ=255) gagGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAc < 1:308268/90‑1 (MQ=255) gCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGGATTTCTCCAGGGCGTCGGCAAACTGa > 2:58320/1‑90 (MQ=255) gCGGGGGTCTCTTCAAGCGGTTTTTGAGGGGAACGGCTGAGGGCGGGTGGCCGACCGGCCGGGGATTTTTCCCGGGCGTCGGCAAAATgc > 1:560906/1‑89 (MQ=255) | GTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGA > NZ_CP009273/4290896‑4291063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |