Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 49,910 T→G W30G (TGG→GGG)  folA → type 3 dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927349,9100TG97.2% 112.3 / ‑5.0 36W30G (TGG→GGG) folAtype 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (13/22);  minor base A (1/0);  total (14/22)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49825‑49996
                                                                                     |                                                                                      
gATCGGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGtt                                                                                    <  2:669225/90‑1 (MQ=255)
     gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTa                                                                                  <  2:419697/87‑1 (MQ=255)
     gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTa                                                                                  >  1:419697/1‑87 (MQ=255)
         gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  2:488984/90‑1 (MQ=255)
          aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          <  2:6232/90‑1 (MQ=255)
          aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          >  1:701759/1‑90 (MQ=255)
              cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      <  1:110648/90‑1 (MQ=255)
              cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      <  2:911456/90‑1 (MQ=255)
              cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      <  2:72868/90‑1 (MQ=255)
               ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCtt                                                                     >  1:55648/1‑90 (MQ=255)
                     aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaataa                                                               <  2:746281/90‑1 (MQ=255)
                          tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCg                                                          >  1:112577/1‑90 (MQ=255)
                                gcgTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCA‑‑ACCTTAAATAAACCCGTGATTATg                                                  >  2:641955/1‑90 (MQ=255)
                                   ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                 >  2:55443/1‑90 (MQ=255)
                                   ttATCGGCATGGAAAACGCCATGCCGGGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                 <  1:892372/90‑1 (MQ=255)
                                    tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                                <  2:224375/90‑1 (MQ=255)
                                    tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                                <  2:183544/90‑1 (MQ=255)
                                    tATCGGCATGAAAAACGCCATGCCGGGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                                <  1:526635/90‑1 (MQ=255)
                                     aTCGGCATGGAAAACGCCATGCCGGGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                               <  1:782327/90‑1 (MQ=255)
                                          cATGAAAAACGCCATCCCGTAAAACCTCCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAAAAAACCCGTGATTATGGGCCGCCa                                          <  1:639226/90‑1 (MQ=255)
                                           aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      >  1:117051/1‑61 (MQ=255)
                                           aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      <  2:117051/61‑1 (MQ=255)
                                           aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  2:112577/90‑1 (MQ=255)
                                            tgaaaaaCCCCATGCCGTGAACCCTCCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa                                        <  1:778774/87‑1 (MQ=255)
                                                   cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           >  2:207382/1‑48 (MQ=255)
                                                   cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  1:207382/48‑1 (MQ=255)
                                                      cATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCATCACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatc                              >  1:409111/1‑90 (MQ=255)
                                                             tGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt                       >  1:409898/1‑90 (MQ=255)
                                                                   ctgcctgcCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg                 >  1:37717/1‑90 (MQ=255)
                                                                   ctgcctgcCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg                 >  1:372819/1‑90 (MQ=255)
                                                                    tgcctgccGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  1:552784/1‑90 (MQ=255)
                                                                            gATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                                                         >  2:48899/1‑41 (MQ=255)
                                                                            gATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                                                         <  1:48899/41‑1 (MQ=255)
                                                                                  gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  2:55648/90‑1 (MQ=255)
                                                                                  gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  1:397643/90‑1 (MQ=255)
                                                                                  gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  2:695145/90‑1 (MQ=255)
                                                                                     |                                                                                      
GATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49825‑49996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: