Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,393	A→T	D6V (GAT→GTT)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,393	0	A	T	100.0%	93.8 / NA	26	D6V (GAT→GTT)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/11);  total (15/11)

TTGACTTATACTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCT  >  NZ_CP009273/1613314‑1613478                                                                                |                                                                                      ttGACTTATACTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAAt                                                                             <  1:789443/90‑1 (MQ=255)         taCTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAattat                                                                     <  2:149860/90‑1 (MQ=255)             tGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCa                                                                 >  1:566784/1‑90 (MQ=255)              gCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCAt                                                                >  2:568401/1‑90 (MQ=255)                  gggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTggg                                                            >  1:555406/1‑90 (MQ=255)                   ggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGt                                                           <  1:124112/90‑1 (MQ=255)                   ggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGt                                                           <  1:680055/90‑1 (MQ=255)                   ggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGt                                                           <  1:11198/90‑1 (MQ=255)                   ggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGt                                                           <  1:256430/90‑1 (MQ=255)                            atCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATc                                                  >  1:684132/1‑90 (MQ=255)                            atCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATc                                                  >  2:527471/1‑90 (MQ=255)                               cccTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCat                                               <  2:272856/90‑1 (MQ=255)                                 cTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCatat                                             >  2:850044/1‑90 (MQ=255)                                 cTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCatat                                             >  1:457613/1‑90 (MQ=255)                                 cTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCatat                                             >  2:546595/1‑90 (MQ=255)                                 cTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCatat                                             >  2:249246/1‑90 (MQ=255)                                   gCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATgg                                           >  1:451283/1‑90 (MQ=255)                                                tGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGt                                                           <  1:436008/61‑1 (MQ=255)                                                tGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGt                                                           >  2:436008/1‑61 (MQ=255)                                                     gggCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATc                         >  1:623264/1‑90 (MQ=255)                                                        cAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGcc                      <  2:801105/90‑1 (MQ=255)                                                               tgtgAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTaa               >  1:812364/1‑90 (MQ=255)                                                                  gAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGa            >  2:141621/1‑90 (MQ=255)                                                                       gTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCat                                               >  1:718410/1‑50 (MQ=255)                                                                       gTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCat                                               <  2:718410/50‑1 (MQ=255)                                                                            aGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGtct  <  1:680635/90‑1 (MQ=255)                                                                                |                                                                                      TTGACTTATACTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCT  >  NZ_CP009273/1613314‑1613478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑