| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,145,167 | 0 | G | T | 60.0% | ‑2.8 / 12.9 | 25 | intergenic (+40/+25) | yegJ/pphC | DUF2314 domain‑containing protein/protein‑serine/threonine phosphatase PphC |
| Reads supporting (aligned to +/- strand): ref base G (8/2); new base T (7/8); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑C‑TGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGC > NZ_CP009273/2145078‑2145240 | ccTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑C‑TGGCGGTTTAAatg > 1:409099/1‑90 (MQ=255) cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑C‑TGGCGGTTGAAttgt < 1:1345306/90‑1 (MQ=255) agaagaatCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑C‑TGGCGGTTGAATTTTTTTCTTCaa > 1:1211674/1‑90 (MQ=255) agaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑C‑TGGCGGTTGAATTGTTGTCTTTaa > 2:517109/1‑90 (MQ=255) agaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑C‑TGGCGGTTGAATTGTTGTCTTCAACCg > 2:1432712/1‑90 (MQ=255) agaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑C‑TGGCGGGTGGATTTTTTTCTTTAAccc > 1:208434/1‑89 (MQ=255) agagAAAAATTATTCCCCAAATCAGCCTTTATATTTATTTTTAAAA‑C‑GGGGGGTTAAATTTTTGTCTTCAACCGCCATGAGAAactcact < 2:409099/90‑1 (MQ=255) tAGTTCTCAATTCAGCTTTTTTTTTTTTTTTTTAAC‑C‑GGGGGGTAAATTTTTTGTTTTAAACCGCCATGAGAAACTCACTCTGCCCACAg < 2:768222/90‑1 (MQ=255) ttCGCTAATCAGCCGTTATATTTATTGTTTAAA‑C‑TGGCGGTTGAATTTTTTTCTTCAACCCCCCTGAGAAAATCACTCTGCCCACAgcgc > 1:879377/1‑90 (MQ=255) tCGCAAATCAGCCTTTATTTTTTTTTTTTAAC‑C‑GGGGGGTTAATTTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGcc < 2:879377/90‑1 (MQ=255) accAGCCTTTATTTTTATTTTTAAAC‑C‑GGGCGTTTAATTTTTTTTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc < 1:517109/88‑1 (MQ=255) tCAGCCGTTATTTTTTTTTTTTAAA‑C‑GGGCGGTTAATTTTTTTTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCa < 2:178732/90‑1 (MQ=255) ccggcGTTTTTTTTTTTTTTTAAATC‑TGGCGGTTGATTTTTTTTTTTAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCa < 2:1245493/86‑1 (MQ=255) aGCCGTTATATTTATTGTTTAAA‑C‑TGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAAc > 2:1315242/1‑90 (MQ=255) cGTTATATTTATTGTTTAAA‑C‑TGGCGGTTGAATTTTTTTTTTCAACCCCCAATAGAAAATCACTCTTCCCCCAGCGCCCGGGCCAACGtt > 1:1356318/1‑90 (MQ=255) gTTTTATTTTTTTTTTAAA‑C‑GGGCGGTTGAATTTTTTTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGttt < 2:609881/90‑1 (MQ=255) tttttttttttAAA‑CGGGGGGGTTAATTTTTTTTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatc < 2:1265001/89‑1 (MQ=255) tattTTTAAAC‑C‑GGGTGGTTGATTTGTTCTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatca < 1:966499/90‑1 (MQ=255) tattGTTTAAA‑C‑TGGCGGTTGAATTGTTGTCTTCAACCGCCCTGAGAAACTCACCCTCCCCACAGAGCCAGTGCCAACGTTTTattatca > 2:1247910/1‑90 (MQ=255) ttGTTTAAA‑C‑TGGCGGATGAATTTTTGTCTTCAACCGCCATTAGAAAATCACTCTTCCCCCAACGCCCGTGCCAACGTTTTatcattatc > 1:669478/1‑90 (MQ=255) tttAAA‑C‑TGGCGGTTGAATTTTTGTCTTTAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGt > 1:1050943/1‑90 (MQ=255) ttAAA‑C‑TGTCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATcctgt > 1:1467064/1‑87 (MQ=255) ttAAA‑C‑TGGCGGTTTAATTGTTTTCTTCAATCTCCATGAGGAACTCACTCTGCCCACAGCGCCCGTGCCTACGTTTTTTTATCATCCGTa > 1:1083456/1‑90 (MQ=255) ttAAA‑C‑TGGCGGTTGAATTGTTGGCTTCCAACACCATGAGAAAGTGACTCTGCCCACAGCGCCAGTGGCAACGTTTTGTAATCATTCgga > 2:690772/1‑88 (MQ=255) tAAA‑C‑TGGCGGTTGAATTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCCCAACGCCAGGGCCAACGTTTTATCATCATCCGTAc > 1:30870/1‑90 (MQ=255) tAAA‑C‑TGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTAc > 2:582465/1‑90 (MQ=255) aa‑C‑GGGCGGTTAATTTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGc < 2:854020/90‑1 (MQ=255) | CCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑C‑TGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGC > NZ_CP009273/2145078‑2145240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |