Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 49,910 T→G W30G (TGG→GGG)  folA → type 3 dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927349,9100TG98.4% 220.5 / NA 63W30G (TGG→GGG) folAtype 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (19/43);  minor base C (1/0);  total (20/43)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49823‑49996
                                                                                       |                                                                                      
aTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCggg                                                                                      >  2:788791/1‑90 (MQ=255)
aTGATCAGTCTGATTGCGGCGCTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCggg                                                                                      >  2:967587/1‑90 (MQ=255)
    tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATTCCGTGGAACCTGTCTGCCGATCTCGCCGGGTTTa                                                                                  <  1:25734/90‑1 (MQ=255)
    tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTa                                                                                  <  1:1125495/90‑1 (MQ=255)
    tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTa                                                                                  <  2:546951/90‑1 (MQ=255)
     cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTaa                                                                                 >  1:409574/1‑90 (MQ=255)
      aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTaaa                                                                                >  2:606708/1‑90 (MQ=255)
      aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTaa                                                                                 >  2:953584/1‑89 (MQ=255)
      aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTaa                                                                                 <  1:953584/89‑1 (MQ=255)
       gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAAc                                                                               <  2:1624346/90‑1 (MQ=255)
          tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCa                                                                            >  1:1294443/1‑90 (MQ=255)
           gATTGCGGCGTTAGCGGTAGATCGGGTTATGGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  1:1014001/90‑1 (MQ=255)
           gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  1:861626/90‑1 (MQ=255)
           gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  1:990324/90‑1 (MQ=255)
           gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  1:473246/90‑1 (MQ=255)
           gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  2:499595/90‑1 (MQ=255)
           gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  2:1294443/90‑1 (MQ=255)
           gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  2:299740/90‑1 (MQ=255)
           gATTGCGGCGTTACCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  1:40414/90‑1 (MQ=255)
           gATGGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  2:578185/90‑1 (MQ=255)
            aTTGCGGCGTTCGCGGTAGATCGCGTTATGGGCATGAAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          <  1:202945/90‑1 (MQ=255)
            aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGAGTTTAAACGCAac                                                                          <  2:1083106/90‑1 (MQ=255)
                cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      <  2:409574/90‑1 (MQ=255)
                cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      <  1:1518741/90‑1 (MQ=255)
                 ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCtt                                                                     >  2:1562542/1‑90 (MQ=255)
                 ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCtt                                                                     >  1:20251/1‑90 (MQ=255)
                 ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCtt                                                                     >  2:870298/1‑90 (MQ=255)
                     ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATTGCGAGGAAGCTGCCTGCCGATTTCGCCGGGATTAAACGGAACACCTtaaat                                                                 >  2:948486/1‑90 (MQ=255)
                      tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaata                                                                <  1:1562542/90‑1 (MQ=255)
                        gCGGTAGATCGCGTTATCGGCATGAAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaataaa                                                              <  1:342954/90‑1 (MQ=255)
                          ggTAGATCGCGTTATCGGCATGAAAAACCCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAcc                                                            <  1:547290/90‑1 (MQ=255)
                           gTAGATCGCGTTATCGGCATGGAAACCCCCATGCCGGGGACCCTCCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAccc                                                           <  1:492367/90‑1 (MQ=255)
                              gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTg                                                        <  1:870298/90‑1 (MQ=255)
                              gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTg                                                        >  1:196836/1‑90 (MQ=255)
                                tCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGAt                                                      >  1:1063143/1‑90 (MQ=255)
                                  gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCCGCCCATCTCGCCCGGTTTAAAAGCAACACCTTAAATAAACCCCGGATTa                                                    >  1:334972/1‑90 (MQ=255)
                                     ttATCGGCATGGAAACCGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                 <  2:792672/90‑1 (MQ=255)
                                     ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                 >  2:788452/1‑90 (MQ=255)
                                      taccGGAATGAAAAACCCCATGCGGGGAACCCTCCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                                <  1:1530691/87‑1 (MQ=255)
                                       aTCGGCATGAAAACCGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                               <  2:928109/90‑1 (MQ=255)
                                          ggCATGAAAACCGCCATGCCGGGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                            <  1:352134/90‑1 (MQ=255)
                                             aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  2:1314109/90‑1 (MQ=255)
                                              tgaaaaaCGCCATGCGGTGAACCCTCCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa                                        <  1:606708/87‑1 (MQ=255)
                                                 aaaaCGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaataa                                                               <  2:1466552/64‑1 (MQ=255)
                                                 aaaaCGCCATGCCGGGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaataa                                                               >  1:1466552/1‑64 (MQ=255)
                                                 aaaaCCCCATCCGGGGAAACCTGCCTGCCGACCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                     <  1:937149/90‑1 (MQ=255)
                                                  aaaCGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTg                                    <  2:475470/90‑1 (MQ=255)
                                                     cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                                 <  2:20251/90‑1 (MQ=255)
                                                          tGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTaaa                                                                                <  2:1338187/38‑1 (MQ=38)
                                                          tGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTaaa                                                                                >  1:1338187/1‑38 (MQ=38)
                                                          tGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                            >  2:451994/1‑90 (MQ=255)
                                                            ccGGGGAACCTCCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                          <  1:1004834/90‑1 (MQ=255)
                                                              gTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg                        >  2:330567/1‑90 (MQ=255)
                                                                ggACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                      <  2:1041000/90‑1 (MQ=255)
                                                                gaaccctccctgCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                      <  1:1501045/86‑1 (MQ=255)
                                                                 gAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcg                     >  2:68771/1‑90 (MQ=255)
                                                                    cctgcctgCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc                  <  1:1477228/90‑1 (MQ=255)
                                                                      tgcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  2:708789/1‑90 (MQ=255)
                                                                      tgcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  1:358240/1‑90 (MQ=255)
                                                                             cGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAgg         <  1:330567/90‑1 (MQ=255)
                                                                             cGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAgg         >  1:1093681/1‑90 (MQ=255)
                                                                               aTCTCGCCGGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc       <  1:948486/90‑1 (MQ=255)
                                                                               aTCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc       <  2:1047942/90‑1 (MQ=255)
                                                                                    gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  1:1165033/90‑1 (MQ=255)
                                                                                    gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  1:708789/90‑1 (MQ=255)
                                                                                       |                                                                                      
ATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49823‑49996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: