Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273822,4100AT53.8% 5.8 / 16.0 13I100N (ATT→AAT) ybhSABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (6/0);  new base T (0/7);  total (6/7)
Fisher's exact test for biased strand distribution p-value = 5.83e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACC  >  NZ_CP009273/822325‑822483
                                                                                      |                                                                         
gTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAAtttt                                                                       >  1:127321/1‑90 (MQ=255)
                    gtgcggtgGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGa                                                   >  1:202607/1‑90 (MQ=255)
                     tgcggtgGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGAt                                                  >  1:143340/1‑90 (MQ=255)
                      ggggtgGCGTGGGCGCGCTCTATTTGTCCCAAAAACCCCACCGAAAAAACCCCCAGCCCGAAATTTTCCCCCGCCTGCATTTTGGCGATc                                                 <  1:288073/88‑1 (MQ=255)
                       gggtgGCGTTGGCGCCCCCCATCTGTCCCGAAAAACCCCCCGAAATAACCACCAGCCCGCGATTTTTCCCCGCCTGCATTTTGGCGATCa                                                <  2:202607/89‑1 (MQ=255)
                        ggtgGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAg                                               >  2:359940/1‑90 (MQ=255)
                              gTTGGCGCGCTCCATCTTTCCCGAAAAACCCCCCGAAATACCCCCCAGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTg                                         <  2:127321/90‑1 (MQ=255)
                              gTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTg                                         >  1:220973/1‑90 (MQ=255)
                               tgggCGCCCTCCTTTTGTTCCGCAAAACCCCCGGGAATACCCCCCAGGCCGCTATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGa                                        <  2:45002/88‑1 (MQ=255)
                                     cgcTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTa                                  >  1:303563/1‑90 (MQ=255)
                                                           cccccGGAAAAACCCCCCAGCCCGGGATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATg             <  2:303563/87‑1 (MQ=255)
                                                                     aaCCCCCAGCCCGGTATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAAcc  <  2:220973/90‑1 (MQ=255)
                                                                     aaCCCCCAGACCGCAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAAcc  <  1:359940/90‑1 (MQ=255)
                                                                                      |                                                                         
GTCGGTGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACC  >  NZ_CP009273/822325‑822483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: