Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,360,4640GT54.5% 9.3 / 17.1 11C353F (TGT→TTT) arnBUDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (5/0);  new base T (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGCGAGCGTTTTCCCACGCTGTCGTTACCGAATACCGAATGGAATAGCGAACGCATCTGTTCTTTGCCGCTGTTCCCGGATATGACTACCGCCGATGCCGACCATGTCATCACAGCCCTTCAGCAACTCGCAGGACAATA  >  NZ_CP009273/2360406‑2360545
                                                          |                                                                                 
cgcgAGCGTTTTCCCACGCTGTCGTTACCGAATACCGAATGGAATAGCGAACGCATCTGTTCTTTGCCGCTGTTCCCGGATATGACTAcc                                                    >  2:387901/1‑90 (MQ=255)
                    gTCGTTACCGAATACCGAATGGAATAGCGAACGCATCTGTTCTTTGCCGCTGTTCCCGGATATGACTACCGCCGATGCCGACCATGtcat                                >  1:364041/1‑90 (MQ=255)
                       tttACCAAATCCCAAATGGAATAGCAACCGCTTCTTTTTTTTCCCCCTGTCCCGGAATATGACTCCCGCCGATGCCGACCATGTCATcac                             <  1:387901/89‑1 (MQ=255)
                        ttcccgaatccggaagGAAATAGCGAACGCATCTTTTTTTTGCCGCTTTCCCCGGATATGACTACCGCCGATGCCGACCATGTCATcaca                            <  1:149055/87‑1 (MQ=255)
                                  ccgaatGGAATAGCGACCCCCTCTTTTTTTTCCCGTTGCCCCCGGATAGGCCCCCCGCCGATGCCGACCATGTCATCACAGCCCTTCAGc                  <  1:322109/90‑1 (MQ=255)
                                    aaagggAATAGCGAACGCTTCTTTTCTTTGCCGCTTTCCCGGGATATGACTACCGCCGATGCCGACCATGTCATCACAGCCCTTCAGCaa                <  2:364041/86‑1 (MQ=255)
                                         gAATAGCGACCCCTTCTTTTTTTTCCCGCTGTTCCGGGATATGCCTCCCGCCGATGCCGACCATGTCATCACAGCCCTTCAGCAACTCGc           <  1:150764/90‑1 (MQ=255)
                                         gAATAGCGAACGCATCTGTTCTTTGCCGCTGTTCCCGGATATGACTACCGCCGATGCCGACCATGTCATCACAGCCCTTCAGCAACTCGc           >  1:123736/1‑90 (MQ=255)
                                           aTAGCGAACGCATTTTTTTTTTGCCGCTGTCCCCGGATATGCCTACCGCCGATGCCGACCATGTCATCACAGCCCTTCAGCAACTCGCAg         <  2:256103/90‑1 (MQ=255)
                                           aTAGCGAACGCATCTGTTCTTTGCCGCTGTTCCCGGATATGACTACCGCCGATGCCGACCACGTCATCACAGCCCTTCAGCAACTCGCAg         >  2:498043/1‑90 (MQ=255)
                                                  aCGCATCTGTTCTTTGCCGCTGTTCCCGGATATGACTACCGCCGATGCCGACCATGTCATCACAGCCCTTCAGCAACTCGCAGGACAATa  >  2:289639/1‑90 (MQ=255)
                                                          |                                                                                 
CGCGAGCGTTTTCCCACGCTGTCGTTACCGAATACCGAATGGAATAGCGAACGCATCTGTTCTTTGCCGCTGTTCCCGGATATGACTACCGCCGATGCCGACCATGTCATCACAGCCCTTCAGCAACTCGCAGGACAATA  >  NZ_CP009273/2360406‑2360545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑