| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,085,146 | 0 | A | C | 54.5% | 8.9 / 11.1 | 11 | G218G (GGA→GGC) | rsmE | 16S rRNA (uracil(1498)‑N(3))‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (0/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGACCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAA > NZ_CP009273/3085057‑3085231 | gTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGa > 2:369524/1‑90 (MQ=255) ctgATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGACCTCGCGtt < 2:322137/90‑1 (MQ=255) aGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGACCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGc > 1:224145/1‑90 (MQ=255) gATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGACCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGcc > 2:61019/1‑90 (MQ=255) gCCATGACTCCCCGCTATCAATTTACTGATACCCTGTGGGGCCCTCGCTTTTTGCGTACAGAGACACCTGCGCTCACCGCCATTACcgcg < 1:50205/90‑1 (MQ=255) tGATATCCTGTTGGGACCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTggg > 1:63609/1‑90 (MQ=255) cTGTTGGGCCCCCGTTTTTTGCGCAAAGAGCCACCTGCGCCCCCCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACgg < 1:369524/90‑1 (MQ=255) cTGTGGGGCCCTCGCTTTTTGCGAACAGAGACAACTGCCCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACgg < 2:472739/90‑1 (MQ=255) gtggggCCCTCGCTTTTTGCGTACAGAGCCAGCCGCGCCCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGgag < 2:248629/87‑1 (MQ=255) ttGGGCCCTCGCTTTTGGCGTAAAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGgaga < 1:47949/90‑1 (MQ=255) tggggCCCTCGCTTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGgaga < 2:200897/88‑1 (MQ=255) tGGGACCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGagaa > 1:488480/1‑90 (MQ=255) | GTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGACCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAA > NZ_CP009273/3085057‑3085231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |