| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,176,035 | 0 | A | G | 57.1% | 6.5 / 15.2 | 14 | I43V (ATC→GTC) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (1/5); new base G (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAA > NZ_CP009273/3175948‑3176116 | gCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGTc > 2:110663/1‑90 (MQ=255) ccACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCa < 1:49903/90‑1 (MQ=255) ttGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGGCATGTTGctcc > 1:399344/1‑89 (MQ=255) ggCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcatc < 1:409875/90‑1 (MQ=255) cTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGTCATGTTTGTCCTCTCATTTATTGAAATGCTTTCTGCCGCCCCagc > 2:246635/1‑90 (MQ=255) cTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGGCATTTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTagc > 2:380356/1‑90 (MQ=255) gCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGGCATGTTGCTCCTCTCATTTATTGAAAAGGTTTCTGCCGCACTAGCAGCTGAAg > 1:482954/1‑90 (MQ=255) gCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGACATGTTGCTCCTCTCATTAATGGGAAAGCTTTCCGCCGCCCCAGCAGCTGGAg > 1:9335/1‑90 (MQ=255) gCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATg > 1:446334/1‑41 (MQ=255) gCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATg < 2:446334/41‑1 (MQ=255) gCGTTTTCGCTAGGTTTTGCGGCGGGGGTCATGTTGCTCATCTCCTTTATGGAAAAGCTTCCTGCCGCCCCAGCAGCTGAAGGAAAGTCg > 1:27284/1‑90 (MQ=255) tttCGCTAGGTTTTGCGGCGGGGGCCGTGTTGCTCATCTCATTAACAGTAATGCTTTCCCCCGCACCATCTACTGAAGTGATGTCGccgg > 1:333596/1‑88 (MQ=255) tGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg < 1:37009/90‑1 (MQ=255) cggcggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGaa < 2:482954/90‑1 (MQ=255) | GCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAA > NZ_CP009273/3175948‑3176116 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |