| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,571,661 | 0 | A | C | 53.8% | 13.2 / 14.7 | 13 | G142G (GGT→GGG) | gntR | gluconate operon transcriptional repressor GntR |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAT > NZ_CP009273/3571574‑3571744 | cATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATAcc > 1:152936/1‑90 (MQ=255) gctgctTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCa > 2:407417/1‑90 (MQ=255) gctTAAAATTTATAAAACCCGACGGCGATATCAAGGCAGGGCGATTTGCTGCCCATCAGTCCCCCCAGGGGAATCCCCGCCACTTCAatc < 2:344071/90‑1 (MQ=255) acccGAGGGCAATATCAGGGCAGGGCGCCTTGCTGCCCATCATTCCCCCCGCGGAAATCCCCGCCACTTCAACCATCTTTAAGGTGcgcg < 2:420322/88‑1 (MQ=255) cccGACGGCAATATCTGGGCATGGCGTCTTGCTGCCCATCATTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 2:14914/89‑1 (MQ=255) aCGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgtg > 1:234451/1‑90 (MQ=255) gCGATATCAAGGCATGGCGCCTTGCTGTCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTgg < 2:14264/90‑1 (MQ=255) caatatAAAGGCATGGCGATTTGCTGCCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTggg < 2:395212/88‑1 (MQ=255) aataaCAAGGCAGGGCGCCTTGCTGCCCATCAGTCCCCCCACGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGt < 2:131760/89‑1 (MQ=255) ttGCTGCCCATCATTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAgg < 2:496908/90‑1 (MQ=255) gTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTc > 1:470375/1‑90 (MQ=255) tCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGatat > 1:161113/1‑90 (MQ=255) gggAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAt > 2:323556/1‑90 (MQ=255) | CATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAT > NZ_CP009273/3571574‑3571744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |