Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,806,4320AC53.8% 6.7 / 11.5 13T114T (ACA→ACC) coaBCbifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base C (0/7);  total (4/9)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGG  >  NZ_CP009273/3806347‑3806508
                                                                                     |                                                                            
ttCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTg                                                                          >  1:498983/1‑90 (MQ=255)
                               ccGTGTTGCTGCCGGAATGGGGAATGCCCTGGTATCGACGATTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACca                                           <  1:71127/90‑1 (MQ=255)
                               ccGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACca                                           <  2:281172/90‑1 (MQ=255)
                               ccGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACca                                           >  2:400492/1‑90 (MQ=255)
                               ccGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCAAGAACca                                           <  1:140066/90‑1 (MQ=255)
                                 gtgtGGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACcagc                                         <  2:239344/90‑1 (MQ=255)
                                            ggAAGGGCGAATGCCCTGGTATCGACGATTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGt                              <  2:498983/90‑1 (MQ=255)
                                                         cccTGGTATCGACGATTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACgc                 <  2:169941/89‑1 (MQ=255)
                                                              gTATCGAGTTTTTGTCGGGCTCCCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCAt            <  1:261317/90‑1 (MQ=255)
                                                              gTATCGACGTTTTGTCTGGCTCCCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCAt            <  1:80553/90‑1 (MQ=255)
                                                              gTAGCGACGTTTTTTCTGGCTCCCCCCGGGCTGGTAGCGGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCAt            <  1:455833/90‑1 (MQ=255)
                                                                       aTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTagag   >  1:321316/1‑90 (MQ=255)
                                                                        tttGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAgg  >  2:390203/1‑90 (MQ=255)
                                                                                     |                                                                            
TTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGG  >  NZ_CP009273/3806347‑3806508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑