| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,086,494 | 0 | T | G | 53.8% | 3.8 / 10.8 | 13 | Y102S (TAC→TCC) | rhaM | L‑rhamnose mutarotase |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (7/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTG‑TTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCA > NZ_CP009273/4086405‑4086573 | ccGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGt > 2:410027/1‑90 (MQ=255) atGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAActtctttc > 2:168034/1‑88 (MQ=255) aTTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACCTCTTTCAGCTCGc > 2:422665/1‑90 (MQ=255) tCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCgg < 2:459961/90‑1 (MQ=255) ttGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACCTTTTTCAGCTCGCTTCCCACCCGGCTGTTTTTCCGGGt > 1:290938/1‑90 (MQ=255) gCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTG‑TTATCCGGGTTAGCg < 1:168034/90‑1 (MQ=255) ggAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGGAAACACTTCTTGCAGCCCGCTACTCCCCGGGCCG‑GTATTCGGGGTATGGGGc > 1:95102/1‑90 (MQ=255) aGGCATCTCTGGCACATTGGGCAATTACGGCAGGGGAAACACATCTTTCAGCCCCCCACTCCCCCGGCCG‑TTATCCGGGGTAGCGGGCAt > 1:459786/1‑90 (MQ=255) cATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCGCTCCTCCCCCGGCTG‑TTTTCCCGGGTAGGGGGCATAAc > 1:215306/1‑90 (MQ=255) aTCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTG‑TTATCCGGGTTAGCg < 1:76593/81‑1 (MQ=255) aTCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTG‑TTATCCGGGTTAGCg > 2:76593/1‑81 (MQ=255) tGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTG‑TTATCCGGGTTAGCGGGCATAACATCGGt < 2:82036/90‑1 (MQ=255) acaTTGGGCAATTACGGCAGGGAAAAAAATTCTTTCAGCTCGCTACTCCCCCGGCTG‑TTTTCCCGGGTAGCGGGCATAAAATCGGTCata > 1:127360/1‑90 (MQ=255) ttACGGCAGGTAAAACACTTCTTTCAGCTCGCTACTCACCCGGCTG‑TTATCCGGGGTATCGGGCATAAAATCGGGCATATATTTccccca > 2:399870/1‑90 (MQ=255) | CCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTG‑TTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCA > NZ_CP009273/4086405‑4086573 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |