Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,310,410	A→G	intergenic (‑118/+263)	yciG ← / ← trpA	general stress protein/tryptophan synthase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,310,410	0	A	G	100.0%	28.5 / NA	10	intergenic (‑118/+263)	yciG/trpA	general stress protein/tryptophan synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/7);  total (3/7)

GAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGAACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGAT  >  NZ_CP009273/1310331‑1310485                                                                                |                                                                            gAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGGAAATATAttt                                                                   <  1:107839/90‑1 (MQ=255)        gtgtgtCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGGAAATATATTTTGAGGaa                                                            <  1:236897/90‑1 (MQ=255)           tgtCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGGAAATATATTTTGAGGAACAt                                                         <  2:82770/90‑1 (MQ=255)                            aTTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGGAAATATATTTTGAGGAACATTCTGGATATATTAACaa                                        <  1:379110/90‑1 (MQ=255)                              tAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGGAAATATATTTTGAGGAACATTCTGGATATATTAACAAtt                                      <  1:486941/90‑1 (MQ=255)                               aGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGGAAATATATTTTGAGGAACATTCTGGATATATTAACAATTa                                     >  1:106686/1‑90 (MQ=255)                                    cTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGGAAATATATTTTGAGGAACATTCTGGATATATTAACAATTACCTGa                                >  1:247869/1‑90 (MQ=255)                                                         aaGCGCAACGAGAAGATAGAGGGGAAATATATTTTGAGGaa                                                            >  1:230717/1‑41 (MQ=255)                                                         aaGCGCAACGAGAAGATAGAGGGGAAATATATTTTGAGGaa                                                            <  2:230717/41‑1 (MQ=255)                                                                  gagaAGATAGAGGGGAAATATATTTTGAGGAACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGAt  <  2:131585/90‑1 (MQ=255)                                                                                |                                                                            GAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGAACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGAT  >  NZ_CP009273/1310331‑1310485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑