Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,393	A→T	D6V (GAT→GTT)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,393	0	A	T	100.0%	23.8 / NA	8	D6V (GAT→GTT)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (2/6);  total (2/6)

GGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACG  >  NZ_CP009273/1613331‑1613467                                                               |                                                                           gggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTggg                                                 <  1:397941/90‑1 (MQ=255)        attatCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTa                                          <  1:181099/90‑1 (MQ=255)           atCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATc                                       >  2:129980/1‑90 (MQ=255)              cccTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCat                                    <  1:116099/90‑1 (MQ=255)                        aaTTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATc                          <  1:129980/90‑1 (MQ=255)                                                tgAAAAGTACCAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACg  <  1:206910/90‑1 (MQ=255)                                                         ccAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGc            >  1:27077/1‑71 (MQ=255)                                                         ccAGCGTTCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGc            <  2:27077/71‑1 (MQ=255)                                                               |                                                                           GGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACG  >  NZ_CP009273/1613331‑1613467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑