| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 807,177 | 0 | T | G | 55.6% | 14.6 / 20.5 | 18 | V67G (GTG→GGG) | bioC | malonyl‑ACP O‑methyltransferase BioC |
| Reads supporting (aligned to +/- strand): ref base T (0/8); new base G (10/0); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAG > NZ_CP009273/807091‑807254 | tCCACAGCGTAAATACACCCCCGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGAc < 2:321689/90‑1 (MQ=255)tCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGAc < 2:32943/90‑1 (MQ=255) gCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGGCACGCGCAGGGGACgggcct > 2:7145/1‑86 (MQ=255) gaTACACCCACGTACTGGACGCGGGTTGTGGGCCTGGCTGGATGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtc < 2:108284/89‑1 (MQ=255) acCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGGCGGGCTTTGATCTCTcg > 2:73762/1‑90 (MQ=255) acCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTcg > 1:151943/1‑90 (MQ=255) cTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACCCGCCGGGGACGGCCCTAGATCTCTCcccccccaag > 2:144242/1‑86 (MQ=255) ggACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCGCGCAGGGGGCGGCCCTAGATCTCTCCCCGCCCAAGCt > 2:172333/1‑90 (MQ=255) ggACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGTCACCCGCCGGGGGCGGCCTTAGATCTCTCCCCCCCaaaggt > 1:380954/1‑86 (MQ=255) ttGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGc < 1:391470/90‑1 (MQ=255) tgtgGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGGCGGCCCTAGATCTCTCCCCGCCCATGCTTGTTTAggga > 2:123748/1‑88 (MQ=255) ctggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGa < 1:73762/90‑1 (MQ=255) tggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCCCCGCCCATGCTTGTTTAGGCACGCCAGaa > 2:104458/1‑90 (MQ=255) tggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGAt < 2:297551/90‑1 (MQ=255) ggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCCCCAATGCTTGTTCAGGCACGCCAGAAGGATg > 1:202389/1‑90 (MQ=255) aGCCGCCACTGGCGGGAAAGTCACGCGCAGGGGACGGCCTTAGATCTCTCCCCGCCAATGCTTTTTCAGGCACGCCCGAAGGATGCCGCa > 1:417382/1‑90 (MQ=255) ggCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATc < 1:475776/90‑1 (MQ=255) gTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGgag < 2:417382/90‑1 (MQ=255) | TCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAG > NZ_CP009273/807091‑807254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |