| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,678,659 | 0 | A | C | 57.1% | 9.6 / 14.8 | 14 | Y70D (TAT→GAT) | glyA | serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCC > NZ_CP009273/2678575‑2678732 | cGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGc > 2:201737/1‑90 (MQ=255) gCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAAccgc > 1:163123/1‑90 (MQ=255) aCGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATAGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGtagtag > 1:206131/1‑90 (MQ=255) gTAGTCGGCGCCAAACTTTTCTTCCCCACGATCGATCGCCATTTTTTCAACAATACCAACATCCCCGCAACCGCCGTAGTAGCGTTTGcc < 2:168569/90‑1 (MQ=255) gAACAGTTCTTCCGCACGATCGACCCCCATTTTTTCAACGATATAAACATCCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTc < 1:101064/90‑1 (MQ=255) agaCATTTCTTCCCCACGATCGATCGCCGTTTGTTCAACGAAATCAACATCCCCGAAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTc < 1:328286/88‑1 (MQ=255) ttCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATttg > 1:338928/1‑90 (MQ=255) cGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATttgct > 2:328286/1‑88 (MQ=255) cgcccgaccgCCATTTTTCCAACAATAAAAACATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 1:302493/86‑1 (MQ=255) cgatcgtcCGCCGTTTGTCCAACAATACCAACATCCTCGCACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 2:358839/90‑1 (MQ=255) cgatcgatCGCCTTTTGTACAAAAATAAAAACATCCCCACAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 2:338928/90‑1 (MQ=255) cgCCAGTTTTACAACAATACAAACATCCTCGCACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTgaga < 2:427126/90‑1 (MQ=255) gttCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTgcg > 1:170173/1‑90 (MQ=255) tCAACAATATCAACATCCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGcc < 1:202669/90‑1 (MQ=255) | CGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCC > NZ_CP009273/2678575‑2678732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |