| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,406,276 | 0 | A | C | 54.5% | 8.8 / 11.8 | 11 | G183G (GGT→GGG) | acrS | multidrug efflux transporter transcriptional repressor AcrS |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATC > NZ_CP009273/3406206‑3406347 | ttttttCTTCTGGAAAAAACTTTCTTAATCCTTTACCGCCCAGGGGGGGGTTTTTTTTAAAAAAATAATACCCCGCCATATTCATTAAcc < 2:77052/88‑1 (MQ=255)ttttttCATCGGGAAAAAACTTTTTTAATACTTTACCGCCCAGGGGGGGGTTTTTTTTAAAAAGACCATACCCCGCCATATTCATTAAcc < 1:221446/88‑1 (MQ=255) ttCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCAGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGa > 1:210777/1‑90 (MQ=255) tAATCCTTTACCGACCGGGGGGGGGTTTTTTTAAAAAAGATAAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGc < 1:155580/90‑1 (MQ=255) ttATCGACGAGGGGGGGGTTTTTTTTAAAAAAAACAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCa < 2:118469/90‑1 (MQ=255) ggggTTTTTTTAAAAAAATCAACCCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAAACATcaca < 2:487133/90‑1 (MQ=255) ggggTTTTTTTAAAAAAATCAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATcaca < 1:380975/90‑1 (MQ=255) ttGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATc > 1:205017/1‑90 (MQ=255) ttGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATc > 1:347735/1‑90 (MQ=255) ttGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATc > 1:37561/1‑90 (MQ=255) ttGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATc > 2:349448/1‑90 (MQ=255) | TGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATC > NZ_CP009273/3406206‑3406347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |