| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,774,215 | 0 | T | G | 68.4% | 1.3 / 11.8 | 19 | G343G (GGT→GGG) | lldD | quinone‑dependent L‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (13/0); total (14/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAG > NZ_CP009273/3774131‑3774303 | cGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTata > 2:22310/1‑88 (MQ=255)cGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGc > 1:36247/1‑90 (MQ=255)cGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGc > 2:157542/1‑90 (MQ=255) gTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCt > 2:162105/1‑90 (MQ=255) gTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGCt > 2:208164/1‑90 (MQ=255) cGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAAc < 2:63884/90‑1 (MQ=255) ttGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGGTTAACTTCTaa > 2:18453/1‑90 (MQ=255) cgcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATc < 1:324072/90‑1 (MQ=255) gcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGGTAACCTGCTAAATCt > 2:427608/1‑90 (MQ=255) cTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTg < 2:5392/90‑1 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGgt < 1:365484/55‑1 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGAt > 1:81480/1‑90 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAAAGAt > 2:156516/1‑90 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGCTAACCTGCTAAATCTGATCGAAAAAGAGAt > 2:133722/1‑90 (MQ=255) gctgGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGt > 1:174119/1‑90 (MQ=255) gTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGTTAAATCTGATCGAAAAAGAGATGAAAggggggg > 2:242359/1‑87 (MQ=255) gTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCCGCTAAATCTGATCGAAAAAGAGATGGAAGGGGCGGTGa > 2:10275/1‑90 (MQ=255) ctGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACt < 1:329906/89‑1 (MQ=255) gCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAAACTGATCGAAAAAGAGATGAAAGGGGGGATGACGCTGACTGGCGCGAAATCg > 2:78644/1‑90 (MQ=255) gCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGc < 1:427608/90‑1 (MQ=255) gtgtAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGGGGCGATGACGCTGACTGGCGCGAAAACGATCAGCGAAAATACGCAAg > 2:115265/1‑90 (MQ=255) gtgtAGCTAACCTGCTAAATCTGATCGAAAAAAAGATGAAAGGGGCGATGACGCTGACTGGCGCGAAAACGATCAGCGAAAATACGCaaa > 2:360819/1‑89 (MQ=255) gtgtAGCTAACCTGCTAAAACTGATCGAAAAAAAGATGAAAGGGGCGATGACGCCGACTGGCGCGAAAACGATCAGCGAAAATACGCAAg > 2:48733/1‑90 (MQ=255) | CGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAG > NZ_CP009273/3774131‑3774303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |