| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,964,457 | 0 | T | G | 53.8% | 6.9 / 14.3 | 13 | V322G (GTG→GGG) | wecB | UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing) |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (7/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTATGAGCCGC > NZ_CP009273/3964370‑3964545 | ttCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGc < 1:409152/90‑1 (MQ=255) gaagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGGCTGGtg > 2:34395/1‑89 (MQ=255) gcgcCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGc < 1:476105/90‑1 (MQ=255) gcCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCAc < 2:272398/90‑1 (MQ=255) ccTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACg < 1:66760/90‑1 (MQ=255) ttCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGa < 1:118850/90‑1 (MQ=255) tGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACCGGTCGGCTTGGTGGGAAGGATAAACACGGAATTATCGAGGAAgggg > 1:362376/1‑87 (MQ=255) ccACTGAGCGTCCGGAAGCGGTGACGGCGGGGATGGGGCGGCGGGGAGGGACGGGGAAGAAGCGGAATGGGGAGGGAGGGAGGCGGCttt > 2:379695/1‑90 (MQ=255) gAGCGTCCGGAAGCGGTGACGGCGGGTACGGGGCGTCTGGGAGGCACGGGTAAGCAGCGAATTGTCGAGGAAGGGACGCGTCTTTTtaaa > 1:129034/1‑90 (MQ=255) gAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGTCTGGGAGGGACGGGTAAACAGCGGAATGTTGGGGGAGGGACGCGGCTTTTtaaa > 2:187815/1‑90 (MQ=255) aaGCGGTGACGGCGGGTACGGGGGGTCTGGTAGGCACGGATAAGCAGCGAATTTTCGGGGGAGGGACGCGGCTTTTTAAAAACGaaaaag > 1:311011/1‑88 (MQ=255) ggTGACGGCGGGTACGGGGCGTCTGGTAGGCACGGATAAACAGCGAAATGTCGGGGAAGTGACGCGGCTTTTTAAAaaagaaaaagaaaa > 1:235249/1‑88 (MQ=255) gTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTATGAGCcgc < 2:458135/90‑1 (MQ=255) | TTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTATGAGCCGC > NZ_CP009273/3964370‑3964545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |