| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,086,499 | 0 | C | A | 53.3% | 8.6 / 24.0 | 15 | V100V (GTG→GTT) | rhaM | L‑rhamnose mutarotase |
| Reads supporting (aligned to +/- strand): ref base C (1/6); new base A (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAA > NZ_CP009273/4086424‑4086583 | tAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTGCAGCTc > 1:202104/1‑90 (MQ=255) tCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAAAACTTCTTGCAGCTCGCTACTc > 1:27198/1‑90 (MQ=255) aaaTTTAAAATATTGTGCCGGAGGCATCTCTGGTACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCt < 2:392179/90‑1 (MQ=255) aaaTTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCt < 2:27198/90‑1 (MQ=255) gCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCg < 1:171854/90‑1 (MQ=255) gCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCg < 1:407149/90‑1 (MQ=255) gCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCg < 2:91165/90‑1 (MQ=255) ggAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGc > 1:209835/1‑90 (MQ=255) cATCTCTGGCACATTGGGCAATTACGGCAGGGGAAAAACTTCTTTCAGCTCGCTACTCACCCGGCCGTTTTCCCGGGTAGCGGGGATaaa > 2:407149/1‑89 (MQ=255) cATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTGCAGCTCCCCACTCACCCGGCTGTTATCCGGGGTAGCGGGCATAAc > 2:168981/1‑90 (MQ=255) tctcTGGCACATTGGGCAATTACGGCAGGGGAAAAACTTCTTGCAGCTCCCTTCTCACCCGGGGGTTTTCCGGGGTTGCGGGCGTAACaa > 2:302610/1‑89 (MQ=255) tctcTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCCGCCCCCTACTCACCCGGCTGTTTTTCCGGGTAGCGGGCATAACAt > 2:5030/1‑90 (MQ=255) caTTGGGCAATTACGGCAGGTAAAAAACTTCTTTCAGCTCCCTACCCACCCGGCTGTTTTCCGGGGTAGCGGGGATAACATCGGTCatat > 2:400433/1‑90 (MQ=255) ttGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCCCTACTCACCCGGCTGTTTTTCGGGGTAGCGGGCATAACATCGGGCatatat > 2:9437/1‑90 (MQ=255) tAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCaa < 2:209835/90‑1 (MQ=255) | TAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAA > NZ_CP009273/4086424‑4086583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |