| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,871 | 0 | T | G | 56.2% | 12.4 / 17.4 | 16 | V35G (GTG→GGG) | ampE | beta‑lactamase regulator AmpE |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (9/0); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGG > NZ_CP009273/115786‑115955 | ttACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGaaa > 1:419265/1‑90 (MQ=255)ttACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGaaa > 2:378991/1‑90 (MQ=255) tgtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAAttttt > 1:436379/1‑90 (MQ=255) tgtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAAttttt > 1:379933/1‑90 (MQ=255) gtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAAtttttt > 2:2635/1‑89 (MQ=255) cGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGgcg < 2:352771/90‑1 (MQ=255) aaGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATg < 1:410172/90‑1 (MQ=255) ggCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCCGGGGCCAGTTTGGCATGACCctt > 2:410172/1‑90 (MQ=255) ggCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAATTTTTTTCTCGGGCGCACGTTTGGCATGACCatt > 1:122323/1‑90 (MQ=255) tGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATg > 1:126953/1‑90 (MQ=255) cTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTg < 1:378991/90‑1 (MQ=255) gAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTa < 1:133729/90‑1 (MQ=255) gTTCTTTCGGCGGGGGAAACATTTTTTTCTCCGGCGCCCGGTAGGCATGACCATTATTGCGATGGGGGGGGCTTTTTTTCTGTTTCgcgc > 2:103115/1‑90 (MQ=255) cggcggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAg < 1:37872/90‑1 (MQ=255) cggcggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAg < 2:419265/90‑1 (MQ=255) gcggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAggg < 1:469810/90‑1 (MQ=255) | TTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGG > NZ_CP009273/115786‑115955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |