| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 701,032 | 0 | A | G | 61.5% | 3.7 / 11.7 | 13 | T545A (ACA→GCA) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base G (8/0); total (10/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGT > NZ_CP009273/700945‑701112 | ccTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGAAAACGACAGAAAAAAAAGTCGTATTACCAGCCGCAGGGGCa > 2:134246/1‑90 (MQ=255) cTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGAAAACCACAGAAAAAAAAGTCGTATCAACAACCGCAGGGGCaa > 2:244001/1‑90 (MQ=255) aaGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGGAAACCACAGATAAAAAAGGCGGATCACCCGCCGCAGGGGCAAACGTGa > 2:436791/1‑90 (MQ=255) cATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAAACGACAGAAAAAAACGTCGTATCACCAGCCGCAGGGGCAAACGTGaaaa > 1:361378/1‑90 (MQ=255) gCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTc < 2:407511/90‑1 (MQ=255) gCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAAACGTCGTATCACCAGCCGCAGGGGCAATCGTGAAAATCTTCAaca > 1:3224/1‑90 (MQ=255) gCGGTGGGTGACGGTGTGGCGGTGAAAACGACAGATAAAAACGTCGGATCACCAGCCGCAGGGGGAAACGTGGAAAACTTTAACaccaac > 2:57879/1‑90 (MQ=255) gtgtGGCGGTGAAAACGACAGAGAAAATCGTCGTATCACCAGCCGCAGGGGCAAAAGTGAAAATCTTTAAAACCAACCACGCGGTCTGcc > 1:323571/1‑90 (MQ=255) cGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGaaa < 1:436791/90‑1 (MQ=255) gAAAACGACAGATAAAAACGTCGTATCACCAGCCGCAGGGGCAATCGTGAAAAACTTCAAAACCAACCACGCGTTCTGCCTGGAAAACGa > 2:168594/1‑90 (MQ=255) tcgtATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGAtcgtcg > 1:330912/1‑90 (MQ=255) tcgtATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGAtcgtcg < 1:452801/90‑1 (MQ=255) gCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGt > 2:161022/1‑90 (MQ=255) | CCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGT > NZ_CP009273/700945‑701112 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |