| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NZ_CP009273 | = 4331930 | 62 (1.380) | 6 (0.130) +CCA |
3/146 | 3.2 | 8.8% | coding (203/1356 nt) | melA | alpha‑galactosidase |
| ? | NZ_CP009273 | 4331931 = | 64 (1.350) | coding (204/1356 nt) | melA | alpha‑galactosidase | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
TGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATG‑AACCTTGCACGGTGAC > NZ_CP009273/4331843‑4332017 | gggccATTGCCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑cc < 2:995538/86‑1 (MQ=255) ggACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑Cca > 2:1469380/1‑90 (MQ=255) ggACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑Cca < 2:524232/90‑1 (MQ=255) aCATTGACCCCACCCGCCTGGAATAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑Ccaca > 1:959939/1‑90 (MQ=255) aCATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑Ccaca > 1:782696/1‑90 (MQ=255) ctttGCCCCCCCCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCacac < 2:193499/88‑1 (MQ=255) ttGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGACAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACAccc > 2:703642/1‑90 (MQ=255) gACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCaa > 1:1387373/1‑90 (MQ=255) gACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCaa > 1:529208/1‑90 (MQ=255) ccccccccGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACa < 1:1403723/90‑1 (MQ=255) ccccccccGCCGGGAAGAGTCGCATTTTGTGGTGCGAAAGCTTATGGATTCAGCGGGGGCCAGCGGCAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACa < 1:1469380/90‑1 (MQ=255) cccACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACag > 1:1412990/1‑90 (MQ=255) ccACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACaga > 2:583446/1‑90 (MQ=255) cACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACagaa > 1:626306/1‑90 (MQ=255) cACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACagaa > 2:1077552/1‑90 (MQ=255) aaGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTag < 2:1387373/90‑1 (MQ=255) agagTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACagaaaga < 1:561321/81‑1 (MQ=255) agagTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTaga < 2:856035/90‑1 (MQ=255) agagTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACagaaaga > 2:561321/1‑81 (MQ=255) gagTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTagag < 2:372974/90‑1 (MQ=255) gTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACagaaagaa < 1:955699/79‑1 (MQ=255) gTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTGCCACCACACCCAACAGAAAGAAGCCTTaga > 1:822698/1‑90 (MQ=255) gTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACagaaagaa > 2:955699/1‑79 (MQ=255) gTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAaagaag > 2:854974/1‑80 (MQ=255) gTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAaagaag < 1:854974/80‑1 (MQ=255) gTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCt < 2:1162766/83‑1 (MQ=255) gTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCt > 1:1162766/1‑83 (MQ=255) gTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGa > 2:227729/1‑90 (MQ=255) gTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGa > 1:85648/1‑90 (MQ=255) tCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGAt > 1:1294463/1‑90 (MQ=255) gtggtgCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGAtttt > 2:31959/1‑90 (MQ=255) gtgCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTGCCACCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGAtttt < 2:822698/90‑1 (MQ=255) gtgCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTc < 2:1294463/90‑1 (MQ=255) cGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCgtg < 1:971114/90‑1 (MQ=255) gaaaGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCgtgg > 2:323393/3‑90 (MQ=255) gTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCgtgg > 1:1307503/1‑90 (MQ=255) gTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCgtgg > 2:125464/1‑90 (MQ=255) tAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCgtggt < 2:581231/90‑1 (MQ=255) aGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAATCCTTAGAGGAt > 1:1252901/1‑71 (MQ=255) aGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAATCCTTAGAGGAt < 2:1252901/71‑1 (MQ=255) aGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGtggtgg > 1:563198/1‑90 (MQ=255) tGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTc < 2:1307503/90‑1 (MQ=255) tGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTc < 1:323393/90‑1 (MQ=255) ggATTCAGCAGGGGCCGGCGGAAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCa < 1:148878/90‑1 (MQ=255) tCAGCAGGGGCCAGCGGCAAAATCACCTGCCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGCCGTGGGGGCATTTCa > 1:622294/1‑90 (MQ=255) cagcagGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTg > 2:675018/1‑90 (MQ=255) gcgggggCCAGCGGCAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGc < 1:141854/87‑1 (MQ=255) gcagGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGc < 2:959939/90‑1 (MQ=255) gcagGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGc < 2:858113/90‑1 (MQ=255) gcagGGGCAAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCAAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGc < 2:107961/90‑1 (MQ=255) ggggCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGt > 1:149624/1‑90 (MQ=255) ggggCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGt > 2:1039614/1‑90 (MQ=255) ggCCAGCGGCAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTc < 2:476914/77‑1 (MQ=255) ggCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTc > 1:476914/1‑77 (MQ=255) ggCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTa > 2:1396073/1‑90 (MQ=255) ggCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTa > 1:440068/1‑90 (MQ=255) ccAGGGGAAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATg < 1:227729/90‑1 (MQ=255) cAGCGGCAAAATCC‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGc < 1:1177127/82‑1 (MQ=255) cAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGc > 2:1177127/1‑82 (MQ=255) aGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATG‑aa < 1:1039614/90‑1 (MQ=255) aGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGAATTTCAGATTGGCGGTTATG‑aa < 2:239160/90‑1 (MQ=255) cAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATG‑AACCTTg > 2:828349/1‑90 (MQ=255) cAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATG‑AAACttt > 1:1407221/1‑89 (MQ=255) aaaaTCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGGTTTT‑AACCTTGc > 1:381186/1‑90 (MQ=255) aaTCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATG‑AACCTTGCAc < 2:440068/90‑1 (MQ=255) aaTCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATG‑AACCTTGCAc < 1:675018/90‑1 (MQ=255) aTCC‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATG‑AACCTTGCACg < 2:584300/90‑1 (MQ=255) cA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTGTGAAACCTTGccggg > 2:48376/1‑86 (MQ=255) cccTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATG‑AACCTTGCACGGTg < 1:147748/89‑1 (MQ=255) cTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATG‑AACCTTGCACGGGGAc > 2:450328/1‑90 (MQ=255) | TGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCA‑‑‑‑CCTG‑‑‑CCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATG‑AACCTTGCACGGTGAC > NZ_CP009273/4331843‑4332017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |