| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 349,999 | 0 | T | G | 41.7% | 60.0 / 31.5 | 72 | V528G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (7/35); new base G (30/0); total (37/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC > NZ_CP009273/349915‑350088 | ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAg < 1:621489/90‑1 (MQ=255)ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAg > 1:1480531/1‑90 (MQ=255)ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAg > 1:417319/1‑90 (MQ=255) gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGCGGGGGGGAAGaa > 1:860453/1‑89 (MQ=255) gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGACGAAGTGGCGGTGGGTGGGGGGaaaaaa > 2:1378764/1‑87 (MQ=255) gggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaaa < 1:1224056/89‑1 (MQ=255) ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGGGGGGGGGAAGATGGGGTGGAAg > 1:566604/1‑90 (MQ=255) tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGc < 2:360608/90‑1 (MQ=255) aCGCTCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCCGAAAGGGCAgggg > 1:58372/1‑88 (MQ=255) cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCTGAAAGGGCAggggg > 2:350084/1‑90 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGGGCTGAAAAGGCAggtggc > 2:489629/1‑90 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCTGAAAGGGCAgggggg > 2:1224056/1‑89 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGCTGGGGGGGAAGATGGGCTGAAAAGGGAgggggg > 2:368710/1‑89 (MQ=255) cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCTGGAAGGGGAggggggg > 1:1009405/1‑88 (MQ=255) cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGGAAGGGGAggggggg > 1:350130/1‑88 (MQ=255) cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGAAAGGGCAggggggg > 2:1182345/1‑88 (MQ=255) gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg < 1:368710/90‑1 (MQ=255) aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGgtggcggtggcg < 1:489629/90‑1 (MQ=255) tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGAGtt < 1:520503/90‑1 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc < 1:1182345/90‑1 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGAAAGGGCAGGGGGGGGGGGGGTTTGTCAt > 2:485496/1‑90 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCAt > 2:1469329/1‑90 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGCGCCGGAAGGGGGGGGGGGGGGGGGGGTTGTccg > 1:419713/1‑88 (MQ=255) gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGc < 1:900431/66‑1 (MQ=255) gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGc > 2:900431/1‑66 (MQ=255) tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTTATTTCGa > 2:830398/1‑90 (MQ=255) ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa < 2:1452765/90‑1 (MQ=255) ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa < 1:834609/90‑1 (MQ=255) ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag < 2:58372/90‑1 (MQ=255) ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGGTGAAAGGGGGGGGGGGGGGGGGGGTTTTCTTTTCGAAag > 2:551903/1‑90 (MQ=255) cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAaga < 2:251226/90‑1 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAagag > 2:834609/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGAAAGGGGGGGGGGGGGGGGGGGTTGTTCTTTCGGAagag > 1:146754/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAagag > 2:1376657/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCCGAAAGGGGAGGGGGGGGGGGGGGTTGTTATTCCGAAagag > 1:757893/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCg < 2:982676/90‑1 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGGGCTGAAAAGGGAGGGGGGGGGGGGGGTTGTCCTTTCGCAAGAGAGCg > 2:972949/1‑90 (MQ=255) aTCCGGCCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTGATTTCGGAagagaggg > 1:766142/1‑88 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:1199624/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:156257/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:381574/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:931914/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:1457770/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGATGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:521832/90‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 1:853017/90‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 2:419713/90‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGAAAAAGAGCGACAg > 2:1463886/1‑90 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAg > 2:875405/1‑90 (MQ=255) cGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGAAGCGCTGAAAAGGGGGGGGGGGGGGGCGGTTGTCCTTCCAAAAGAGAGCGCCAGtgt > 2:1342709/1‑88 (MQ=255) ttGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg < 1:1469329/90‑1 (MQ=255) tGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGa < 2:146754/90‑1 (MQ=255) gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa < 1:349935/38‑1 (MQ=255) gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa < 2:1480531/90‑1 (MQ=255) gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa < 2:766142/90‑1 (MQ=255) gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa < 2:374751/90‑1 (MQ=255) gCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGaa > 2:349935/1‑38 (MQ=255) gCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCCGGAAGGGGAGGGGGCGGGGGGGTTTGTCATTCCCAAAGAGAGCGGCAGGCTggga > 2:381574/1‑88 (MQ=255) aaGTGGCGGTGGGTGGGGGGGAAGAGGGGGTGGAAGGGCGGGGGGCGGGGGGGGTTGTTCTTCCGCAAGAGAGCGACAGTCTGGAAGagt > 1:437562/1‑88 (MQ=255) ggCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGAAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGa < 2:757893/90‑1 (MQ=255) ggCGGTGGTTGGGGGGAAAAATGCGCTGAAAAGGCAGGTGGCGGGGGCGTTTATCATTTCGAAAGAGAGCGACAGTCTGGAAGAACGTGa > 2:1179623/1‑90 (MQ=255) cGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGGAGATCGTGAtg > 2:1058169/1‑90 (MQ=255) ttGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcgc < 2:103019/90‑1 (MQ=255) tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 2:528350/90‑1 (MQ=255) tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 2:641575/90‑1 (MQ=255) tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCAAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 1:1378764/90‑1 (MQ=255) ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:765372/63‑1 (MQ=255) ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc > 2:765372/1‑63 (MQ=255) ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 1:923278/89‑1 (MQ=255) ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa > 2:923278/1‑89 (MQ=255) ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc < 2:1324999/90‑1 (MQ=255) ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGAAc > 1:1061040/1‑90 (MQ=255) gggTGAAAGATGCGCTGAAAGGGCCGGTGGCGGTGGCGTTTGTCCTTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGCGGCGCACt > 2:19616/1‑90 (MQ=255) tGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc > 1:437947/1‑90 (MQ=255) | TTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC > NZ_CP009273/349915‑350088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |