| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 595,032 | 0 | T | G | 40.0% | 3.2 / 14.2 | 21 | V288G (GTG→GGG) | cusA | Cu(+)/Ag(+) efflux RND transporter permease subunit CusA |
| Reads supporting (aligned to +/- strand): ref base T (1/11); new base G (8/0); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGCAAAAACGCCC‑GAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCG > NZ_CP009273/594946‑595117 | gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGggtggt < 1:1075550/90‑1 (MQ=255) ttGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGGGGGGGTCCTGc > 2:889645/1‑90 (MQ=255) gCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGggt < 1:721285/77‑1 (MQ=255) tCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGCa < 1:570740/90‑1 (MQ=255) aGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGCaaaa < 2:593035/90‑1 (MQ=255) tGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGG‑GGGGGGGGGGGCCTCCGGGCCGGCGAAAAAGCCC‑CAGAAgggg > 1:1331330/1‑87 (MQ=255) tGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGG‑GGGGGGGGGGGCCCCCGGTCCGGCAAAAACACCC‑GAGAAGTGa > 1:1381866/1‑90 (MQ=255) tGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGGGGGGGTCCTGCGGTCCCGCAAAAAAGCCC‑GAGAAGTGa > 1:520728/1‑90 (MQ=255) gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGGGGGGGTCCTTCGGTCCGGCAAAAAAGCCC‑CAGGAGGGAt > 2:692887/1‑90 (MQ=255) cgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGCAAAAACGCCC‑GAGAAGTGATc < 1:692887/90‑1 (MQ=255) ccGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGCAAAAACGCCC‑GAGAAGTGATcgc < 1:947229/90‑1 (MQ=255) ccGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGCAAAAACGCCC‑GAGAAGTGATcgc < 2:69477/90‑1 (MQ=255) gCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGG‑GGGGGGGGGGTCCTGCGGTCCGGCAAAAAAGCCC‑GAGAAGGGATGGccgccg > 1:1057141/1‑90 (MQ=255) ttGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGAAAAAACGCCC‑GAGAAGTGATCGCCGCCGTGa < 1:360195/90‑1 (MQ=255) tGCCGAACTAAACGGCGAAGGCGAAGGGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGCAAAAACGCCC‑GAGAAGTGATCGCCGCCGTGaa < 1:1298450/90‑1 (MQ=255) gCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGGTCCTGCGATCCGGCAAAAAAGCCC‑GAGAAGGGGTCGccgccgggga > 2:133189/1‑86 (MQ=255) aaCTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGCAAAAACGCCC‑GAGAAGTGATCGCCGCCGTGAAGGACa < 1:1463033/90‑1 (MQ=255) gcgaaggcgaagTGGCGGGG‑GGGGGGGGGGTCCTGCGATCCGGCAAAAAAGCCC‑GGGAAGGGATCGCCGCCGGGAAGGGCAAACAGGaaa > 1:265630/1‑90 (MQ=255) aaggcgaagTGGCGGGC‑GGGGCGGGGATCCTGCCATCCGGCCAAAACGCCC‑GAGAAGTGATCGCCGCCGGGAAGGACAAAATGGAAACGc > 2:455883/1‑90 (MQ=255) ggcgaagTGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGCAAAAACGCCC‑GAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTg < 2:1438089/90‑1 (MQ=255) gcgggc‑ggGGTGGTGATCCTGCGATCCGGC‑AAAACGCCC‑GAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTc < 2:939812/90‑1 (MQ=255) cgggc‑gggGTGGTGATCCTGCGATCCGGCAAAAACGCCC‑GAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTc < 2:208360/90‑1 (MQ=255) gggTGGTGATCCTGCGATCCGGCAAAAACGCCCGGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCg > 1:1135335/1‑90 (MQ=255) | GCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGC‑GGGGTGGTGATCCTGCGATCCGGCAAAAACGCCC‑GAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCG > NZ_CP009273/594946‑595117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |