| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 701,009 | 0 | T | A | 50.0% | 12.8 / 12.1 | 22 | I537N (ATC→AAC) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base T (1/10); new base A (11/0); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCG > NZ_CP009273/700925‑701098 | tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGGAAACGACAGAAAAAAacgtcg > 2:837728/1‑90 (MQ=255)tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGGAAACGACAGAAAAAAacgtcg > 1:824638/1‑90 (MQ=255)tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGAAAACGACAGATAAAAaagtcg > 2:25969/1‑90 (MQ=255) cGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGGAAACGACAGAAAAAAAcgacgt > 1:860376/1‑90 (MQ=255) cTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCa < 1:293872/90‑1 (MQ=255) cTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCa < 1:865594/90‑1 (MQ=255) ggATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGGAAACGACAGATAAAAACGTCGTATTACCCGc > 1:563238/1‑90 (MQ=255) tCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAAACGACAGAAAAAAACGTCGTATCACCAGCCGc > 2:570800/1‑90 (MQ=255) aGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGGAACCGACAGAAAAAAAAGTCGGATCACCcgccgcgg > 1:85034/1‑88 (MQ=255) ttCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAAACGTCGTATCACCAGCCGCAgggg > 2:602967/1‑89 (MQ=255) cTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGGAAACGACAGAAAAAAAAGGGGGATCACCACCCCCAGGGGCaa > 1:1340209/1‑90 (MQ=255) gAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGAAAACCACAGAAAAAAAAATCGTATCACCAGCCGCCGGGGCAAACggg > 1:1289136/1‑88 (MQ=255) cATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGaaaa < 1:736115/90‑1 (MQ=255) cATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGaaaa < 2:1289136/90‑1 (MQ=255) aGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACaccaa < 2:824638/90‑1 (MQ=255) aGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACaccaa < 1:570800/90‑1 (MQ=255) gtgggtgACGGTGTGGCGGTGAAAACGACAGAAAAAAAAGTCGTATCACCACCCCCAGGGGCAAACGGGGAAAAATTCAACacccaccaa > 1:385050/1‑89 (MQ=255) gACGGTGTGGCGGGGAAACCCACAGATAAAAACGTCGCATCACCCACCCCAGGGACAAACGGGGAAAACTTTAACACCAACCACCCGTTc > 2:594519/1‑90 (MQ=255) aCGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCt < 1:1005043/90‑1 (MQ=255) gtgtGGCGGGGAAAACGACAGATAAAAAAGGCGTATCACCAGCCGCAGGGGCAAACGGGGAAAAATTTAAAACCAACCCCGCGCTCTGcc > 1:1074799/1‑90 (MQ=255) gtgGCGGTGGAACCGACAGATAAAAAAGTCGTATCACCCGCCGCAGGGGGAAACGGGAAAAAATTCAAAACCAACCACGCCGTCTGcccg > 1:905662/1‑88 (MQ=255) aaCCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGaaa > 2:526877/1‑90 (MQ=255) aCCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGaaaa < 1:1109925/90‑1 (MQ=255) aaaaTCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGgag < 2:860376/90‑1 (MQ=255) aTCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGAtc < 2:139807/90‑1 (MQ=255) tcgtcgTATCACCATCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGAtcg < 1:837728/90‑1 (MQ=255) tcgtcgTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGAtcg < 1:594519/90‑1 (MQ=255) | TCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCG > NZ_CP009273/700925‑701098 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |