| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,213,865 | 0 | G | T | 52.6% | 12.1 / 25.7 | 19 | S361I (AGT→ATT) | pdeG | cyclic diguanylate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (6/3); new base T (0/10); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTG > NZ_CP009273/1213800‑1213950 | tatttttAGCCTTTCGAATTTTTTTCCCCCGACTTTTGTCGACGAGTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCt < 1:756510/87‑1 (MQ=255) ttAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTAC‑‑‑CCGCGATTTAAACCTTGTTCTgg > 1:89794/1‑90 (MQ=255) cTTCGCATATTTTTCCGCCGCCTTTTGTCGACGAGTTTTTAATTTCCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAg < 2:945791/90‑1 (MQ=255) tCGAATATTTTTCCGCCGCCTTTTGTCGAGGGTTTTTTAATTTCCCGTGACTTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTc < 1:41495/90‑1 (MQ=255) ctgCATATTTTTTCGCCGCCTTTTGTCGACGAGTTTTTATTTTTCCGTGACTTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTc < 1:402079/88‑1 (MQ=255) tattTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCg > 1:479010/1‑90 (MQ=255) ttccgccgcCGTTTGTGGAGGATTTTTTAATTTCCCGTGACTTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTg < 1:1269853/87‑1 (MQ=255) tCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAg > 2:1269853/1‑90 (MQ=255) cGCCGTCGTTTGTCGACGAGTTTTAAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 2:479010/90‑1 (MQ=255) cGCCGACGTTTGTCGACGGTTGTTAAATTTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 2:264572/90‑1 (MQ=255) gCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGcc > 2:1190776/1‑90 (MQ=255) gACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCAtt > 2:231094/1‑90 (MQ=255) acttttGTCGAGGAGTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTg < 1:265299/87‑1 (MQ=255) gTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGtt > 2:1406767/1‑90 (MQ=255) ttttAAATTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTc < 2:1054658/89‑1 (MQ=255) tCCGTGACTTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 2:1419742/90‑1 (MQ=255) ccGTGACAGTTTTAC‑‑‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGtt < 2:89794/90‑1 (MQ=255) cGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 2:192385/90‑1 (MQ=255) gACATTTTACCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTg < 2:6387/90‑1 (MQ=255) | TAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTG > NZ_CP009273/1213800‑1213950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |