| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,371,314 | 0 | G | A | 52.9% | 3.1 / 17.7 | 17 | F475F (TTC→TTT) | menD | 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase |
| Reads supporting (aligned to +/- strand): ref base G (6/2); new base A (0/9); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGC > NZ_CP009273/2371226‑2371386 | cGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCgaga > 1:795069/1‑90 (MQ=255)cGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCgaga > 1:862399/1‑90 (MQ=255) ggcggcgTGCTAAAAATGGACGTTTTGCGGCATAAGATAAAACCGCTCCCGCTCGTTTTGGGGCGTGGGCAACAGCGAAAAATTTTcccc < 1:204051/90‑1 (MQ=255) cagggcgTGCTGAAAATGGTTTTTTTGCGGCATCAGATAAAAGCGCTCCCGCACGCTTGGCGGTGTGGGGAAGAGGAAAAAAGTTTGccc < 2:746004/87‑1 (MQ=255) ggcgTGCTCAAAATGGACTTTTTGCGGCATCAGAAAAAACCGCCCCCGCTCGTTTTGCGGGGTGGGAAACAGCGAAAAATTTTGCccgcc < 1:1202111/90‑1 (MQ=255) gCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgttgt > 2:250472/1‑90 (MQ=255) aaaTGGACTTTTTGCGGCATCAGATAAAACCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTc < 2:955526/90‑1 (MQ=255) aaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgttgttgctgt > 1:746004/1‑86 (MQ=255) ggACGTTTTGCGGCATAAAAAAAAACCCCTCCCGCTCGTTTTGCGGCGTTGGCAACAGCAAAAAAATTTCCCCGCCGTTGTTGTTcacca < 1:995029/90‑1 (MQ=255) gACGTTTTGCGGCATCAGATAGAACCCCCCCCGCTCGTTTTGCGGCGTTGGAAACAGCGAAAAAATTTCCCCGCCGTTGTTGTTcaccac < 1:1192544/90‑1 (MQ=255) ggCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATAcc > 2:1180713/1‑90 (MQ=255) tCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAgcg > 2:437370/1‑90 (MQ=255) cccctcgttTTGCGGGGTGGGAAACAGCAAAAAAATTCCCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACg < 1:437370/87‑1 (MQ=255) gctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcacca > 1:1139295/1‑57 (MQ=255) gctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcacca < 2:1139295/57‑1 (MQ=255) tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcaccac > 1:915258/1‑56 (MQ=255) tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcaccac < 2:915258/56‑1 (MQ=255) ggcgTTGGCAACAGCGAAAAATTTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgc < 2:815006/90‑1 (MQ=255) ggcgTTGGAAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgc < 2:570207/90‑1 (MQ=255) | CGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGC > NZ_CP009273/2371226‑2371386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |