Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,267,806	A→G	D137G (GAC→GGC)	soxR →	redox‑sensitive transcriptional activator SoxR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,267,806	0	A	G	100.0%	151.0 / NA	44	D137G (GAC→GGC)	soxR	redox‑sensitive transcriptional activator SoxR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

CCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCC  >  NZ_CP009273/4267719‑4267892                                                                                        |                                                                                       ccTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGcc                                                                                      <  2:789434/90‑1 (MQ=255)   ttAGTGTCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGc                                                                                    <  1:952997/90‑1 (MQ=255)    tAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCt                                                                                   >  1:409801/1‑90 (MQ=255)     aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCtt                                                                                  >  2:522552/1‑90 (MQ=255)     aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCtt                                                                                  <  1:1465464/90‑1 (MQ=255)        ggCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAgg                                                                               <  2:701073/90‑1 (MQ=255)        ggCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGACCGTTGCGTAACCCGGGCGGCCGCTTAgg                                                                               <  2:1175530/90‑1 (MQ=255)          cgcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGgag                                                                             >  2:1312925/1‑90 (MQ=255)           gcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGgaga                                                                            >  1:1056460/1‑90 (MQ=255)             tGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGagaag                                                                          <  2:329187/90‑1 (MQ=255)              gCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGagaaga                                                                         >  2:700459/1‑90 (MQ=255)                    cGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTAc                                                                   <  2:782422/90‑1 (MQ=255)                        cTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCgga                                                               <  2:1118561/90‑2 (MQ=255)                        cTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGgc                                                               >  2:1201436/1‑90 (MQ=255)                               gATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCt                                                        >  2:452667/1‑90 (MQ=255)                                  gTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGc                                                     >  2:1071954/1‑90 (MQ=255)                                    aTTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTATCGGCGCACGCTTGCTg                                                   <  1:795473/90‑1 (MQ=255)                                         ttGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCa                                                            >  2:999474/1‑76 (MQ=255)                                         ttGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCa                                                            <  1:999474/76‑1 (MQ=255)                                         ttGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGa                                              >  1:1001442/1‑90 (MQ=255)                                         ttGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGa                                              >  2:658314/1‑90 (MQ=255)                                         ttGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGa                                              >  2:389399/1‑90 (MQ=255)                                            tgGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGa                                           >  2:1421782/1‑90 (MQ=255)                                             ggCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGaa                                          >  2:1010414/1‑90 (MQ=255)                                              gCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAAc                                         <  1:452667/90‑1 (MQ=255)                                              gCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAAc                                         <  1:389399/90‑1 (MQ=255)                                                 gCCTTTCGCGCAGTGATTGCCCGTTTCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACaaa                                      >  1:693068/1‑90 (MQ=255)                                                     ttCGCGCAGTGATTGCCCGTTTCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTa                                  >  2:1366173/1‑90 (MQ=255)                                                         cgcAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAgc                              <  1:766044/90‑1 (MQ=255)                                                               gATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCt                                                        >  2:1098790/1‑58 (MQ=255)                                                               gATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCt                                                        <  1:1098790/58‑1 (MQ=255)                                                                 ttGCCCGTTTCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAg                      <  2:693068/90‑1 (MQ=255)                                                                 ttGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAg                      <  2:45065/90‑1 (MQ=255)                                                                   gCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACTGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAggg                    <  2:1061047/90‑1 (MQ=255)                                                                   gCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAggg                    <  2:418964/90‑1 (MQ=255)                                                                     ccGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGgcg                  <  1:1201436/90‑1 (MQ=255)                                                                     ccGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGgcg                  >  2:191045/1‑90 (MQ=255)                                                                       gTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTg                                                   >  1:505711/1‑55 (MQ=255)                                                                       gTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTg                                                   <  2:505711/55‑1 (MQ=255)                                                                       gTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAAc                                         <  1:994423/65‑1 (MQ=255)                                                                       gTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAAc                                         >  2:994423/1‑65 (MQ=255)                                                                       gTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCt                >  2:339439/1‑90 (MQ=255)                                                                              aaCCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGGAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgttt         >  1:1103436/1‑90 (MQ=255)                                                                                    ggcggcCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGgc                   >  2:699778/1‑74 (MQ=255)                                                                                    ggcggcCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGgc                   <  1:699778/74‑1 (MQ=255)                                                                                     gCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTcc  <  1:1071954/90‑1 (MQ=255)                                                                                        |                                                                                       CCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCC  >  NZ_CP009273/4267719‑4267892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑