| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,336,492 | 0 | T | G | 61.5% | ‑0.6 / 10.0 | 13 | T218P (ACC→CCC) | fumB | class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base G (8/0); total (10/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGA‑GGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTT‑GGTTTCCTGGTAGAGATACGTTTTGTTGGCAG > NZ_CP009273/4336408‑4336578 | tGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGTACCGAGGGGAAGCa > 2:283839/1‑90 (MQ=255)tGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCGGCAGTACCGAGGGGACGCa > 2:226851/1‑90 (MQ=255) gACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGA‑GGAAGTGTTTTAGt > 2:126006/1‑90 (MQ=255) aCCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGA‑GGAAGTTTTTCAGTTTGcc < 1:283839/90‑1 (MQ=255) cAATCACAAACGCGATATGGTACGGCGGGGAGGGGGCGGTGACGAGGGTACGCGTTTTTTTTACCAGGGAAGTTTTT‑AGCTTGGCggggg > 1:227279/1‑88 (MQ=255) gATATGGTACGGCGGGCAGGGTGCAGTACCCAGGGGACGCATTTTTTCGACGA‑GGAAGGTTTTTAGTTTGCCGGGGGGCAGCAGGGCttt > 2:180022/1‑90 (MQ=255) atGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGA‑GGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTT‑GGt < 1:205637/90‑1 (MQ=255) gcggGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGA‑GGAAGTTTTTTAGTTTGCCGGGGGGCAGCAGGGGTTT‑TGTTTTCTGGt > 2:236902/1‑90 (MQ=255) gggCAGGGTGCAGTACCGAGGGGGCGCATTTTTTCGACGG‑GGGAGGTTTTTAGTTTTTCGGGGGGGAGGAGGGCTTTGGGTTTCTTggag > 1:186598/1‑88 (MQ=255) cAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGA‑GGGAGGTTTTTAGTTTTCCGGGGGGCAGCAGGGGTTT‑TGTTTTCTGGTAgaga > 1:62598/1‑90 (MQ=255) tGCAGTACCGAGGGGACGCATTTTCTCGACGA‑GGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTT‑GGTTTCCTGGTAGAGATACGt > 1:42452/1‑90 (MQ=255) ccGAGGGTACGCATTTTCTCGACGA‑GGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTT‑GGTTTCCTGGTAGAGATACGTTttgttg > 1:61847/1‑90 (MQ=255) gggTACGCATTTTCTCGACGA‑GGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTT‑GGTTTCCTGGTAGAGATACGTTTTGTTGGCag < 2:186598/90‑1 (MQ=255) | TGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGA‑GGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTT‑GGTTTCCTGGTAGAGATACGTTTTGTTGGCAG > NZ_CP009273/4336408‑4336578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |