| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 858,015 | 0 | G | T | 53.8% | 3.8 / 17.3 | 13 | H16Q (CAC→CAA) | ybiW | glycyl radical protein |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base T (7/0); total (10/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCA > NZ_CP009273/857928‑858103 | aTCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCATAATATGCACCAGCGCATTTTtgtg < 2:88653/90‑1 (MQ=255) tCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTgc < 1:257625/90‑1 (MQ=255) tgttgATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATATGCACCAGCGCCTTTTTTTGCGCTTTAATg > 1:16316/1‑90 (MQ=255) cATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATATGCACCAGCGCGTTTTTTTTCGCTTTTATGGGGGCGCt > 2:237142/1‑90 (MQ=255) cGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCg < 1:212340/90‑1 (MQ=255) gTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATATGCACCCGCCCCTTTTTTTGCGCTTTTAAGGGGTCGCCGAGCGGGGCCAg > 1:94370/1‑90 (MQ=255) gcgcgcgcTCGGTACAGACTGGCGGTTTTACAATATGCACCGCCGCATTTTTTTGGGCTTTTATGCGGGCGCTGAGCGTGTTCCGGTTCa > 1:35318/1‑90 (MQ=255) gcgcgcgcTCGGTACAGACTGGCGGTTTTACAATATGCACCAGCGCATTTTTTTGCGCTTTTATGCGGGCGCTGAGCGTGTCCAGTTTCa > 2:69739/1‑90 (MQ=255) gcgcgcgcTCGGTACAGACTGGCGGTTTCACAATATGGACCCGCGCCTTTTTTTGCGCTTTTATGCGGGCGCTGGGCGGGTCCCGTTTCa > 1:127396/1‑90 (MQ=255) gcgcTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAgtgt < 2:226195/90‑1 (MQ=255) aGACTGGCGGTTTTACAATATTCACCAGCGCATTTTTTTGCGCTTTTATGCGGTCGCTGAGCGGGTCCCGTTTTCGTGTGGGCATGgttc > 1:226195/1‑89 (MQ=255) gCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATcc > 2:22455/1‑90 (MQ=255) cAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAAc > 1:159156/1‑90 (MQ=255) aGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAAcc > 2:138686/1‑90 (MQ=255) gtgCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTggca < 1:79370/89‑1 (MQ=255) gtgCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTggca > 2:79370/1‑89 (MQ=255) | ATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCA > NZ_CP009273/857928‑858103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |