| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,125,798 | 0 | A | C | 54.5% | 5.5 / 11.5 | 11 | G174G (GGT→GGG) | flgA | flagellar basal body P‑ring formation chaperone FlgA |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCG > NZ_CP009273/1125734‑1125879 | gcgcATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGtt > 1:192710/1‑90 (MQ=255) gCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGc > 2:83035/1‑90 (MQ=255) gTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTg > 1:305665/1‑90 (MQ=255) gTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTg > 2:79016/1‑90 (MQ=255) tCAGCGCCTGCCCTTCTGGTTTGGCGCAAACCCCACCCCCGCGGGCGATCACATTGGCGGGTTGTCCCGTTTTTCCCCGCCATGCCTGGc < 1:79016/90‑1 (MQ=255) tCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCCCCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGc < 2:97469/90‑1 (MQ=255) tGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTgg > 2:311627/1‑90 (MQ=255) tCTGGGTTGGCGCAAACCCCACCCCCGCGGGCGATCACATTGACGCGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAAc < 1:263950/90‑1 (MQ=255) accccATCCCCGCTGGGGATCACATTGGCGTGTTGTCCCGTTTTCCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg < 1:311627/88‑1 (MQ=255) accccATCCCCGCGGGCGATCACATTGCCGCGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg < 2:305665/88‑1 (MQ=255) accccACCCCCGCTGGCGATCACATTGCCGCGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg < 2:35101/88‑1 (MQ=255) | GCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCG > NZ_CP009273/1125734‑1125879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |